PIWIL4 antibody

Catalog No. ABIN7256712

Product Details anti-PIWIL4 Antibody

Target: PIWIL4 (Piwi-Like 4 (PIWIL4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIWIL4 antibody is un-conjugated

Application: Western Blotting (WB)

Characteristics: Polyclonal Antibody

Purification: Affinity purification

Immunogen: Recombinant fusion protein of human PIWIL4 (NP_689644.2).

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for PIWIL4

Target: PIWIL4 (Piwi-Like 4 (PIWIL4))

Alternative Name: PIWIL4 (PIWIL4 Products)

Synonyms: HIWI2 antibody, MIWI2 antibody, 9230101H05Rik antibody, Miwi2 antibody, piwi like RNA-mediated gene silencing 4 antibody, piwi-like RNA-mediated gene silencing 4 antibody, PIWIL4 antibody, Piwil4 antibody

Background: PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells. Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association.

Molecular Weight:

Observed_MW: 105 kDa

Calculated_MW: 52 kDa/56 kDa/96 kDa

Gene ID: 143689

UniProt: Q7Z3Z4