NSMAF antibody (AA 51-868)

Catalog No. ABIN7601941

The Rabbit Polyclonal anti-NSMAF antibody has been validated for WB, ELISA and FACS. It is suitable to detect NSMAF in samples from Human, Mouse and Rat.

Quick Overview for NSMAF antibody (AA 51-868) (ABIN7601941)

Target: NSMAF (Neutral Sphingomyelinase (N-SMase) Activation Associated Factor (NSMAF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSMAF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-NSMAF Antibody

Binding Specificity: AA 51-868

Purpose: Anti-NSMAF Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-NSMAF Antibody Picoband® (ABIN7601941). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NSMAF recombinant protein (Position: K51-L868). Human NSMAF shares 92.3% amino acid (aa) sequence identity with mouse NSMAF.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Adam-Klages, S., Adam, D., Wiegmann, K., Struve, S., Kolanus, W., Schneider-Mergener, J., Kronke, M. FAN, a novel WD-repeat protein, couples the p55 TNF-receptor to neutral sphingomyelinase. Cell 86: 937-947, 1996. 2. Montfort, A., de Badts, B., Douin-Echinard, V., Martin, P. G. P., Iacovoni, J., Nevoit, C., Therville, N., Garcia, V., Bertrand, M.-A., Bessieres, M.-H., Trombe, M.-C., Levade, T., Benoist, H., Segui, B. FAN stimulates TNF-alpha-induced gene expression, leukocyte recruitment, and humoral response. J. Immun. 183: 5369-5378, 2009. 3. Segui, B., Cuvillier, O., Adam-Klages, S., Garcia, V., Malagarie-Cazenave, S., Leveque, S., Caspar-Bauguil, S., Coudert, J., Salvayre, R., Kronke, M., Levade, T. Involvement of FAN in TNF-induced apoptosis. J. Clin. Invest. 108: 143-151, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NSMAF

Target: NSMAF (Neutral Sphingomyelinase (N-SMase) Activation Associated Factor (NSMAF))

Alternative Name: NSMAF

Background:

Synonyms: NSMAF, FAN, Protein FAN, Factor associated with neutral sphingomyelinase activation, Factor associated with N-SMase activation

Background: This gene encodes a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation. Alternative splicing results in multiple transcript variants.

Molecular Weight: 104 kDa

Gene ID: 8439

UniProt: Q92636