Nth Endonuclease III-Like 1 (NTHL1) (AA 52-312) antibody

Catalog No. ABIN7601953

The Rabbit Polyclonal anti-Nth Endonuclease III-Like 1 antibody is suitable to detect Nth Endonuclease III-Like 1 in samples from Human. It has been validated for WB, ELISA, IF, ICC and FACS.

Quick Overview for Nth Endonuclease III-Like 1 (NTHL1) (AA 52-312) antibody (ABIN7601953)

Target: Nth Endonuclease III-Like 1 (NTHL1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details

Binding Specificity: AA 52-312

Purpose: Anti-NTHL1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NTHL1 Antibody (ABIN7601953). Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NTHL1 recombinant protein (Position: H52-L312).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details

Target: Nth Endonuclease III-Like 1 (NTHL1)

Alternative Name: NTHL1

Background:

Synonyms: Rapamycin-insensitive companion of mTOR, AVO3 homolog, hAVO3, RICTOR, KIAA1999

Tissue Specificity: In fetal tissues, it is highly expressed in heart, lung liver and kidney, and weakly expressed in brain. In adult, it is highly expressed in pancreas, spleen, thymus and peripheral blood leukocytes, expressed at moderate levels in heart, placenta, lung, liver, skeletal muscle, kidney, prostate, ovary, small intestine and colon, and weakly expressed in brain and testis.

Background: Endonuclease III-like protein 1 is an enzyme that in humans is encoded by the NTHL1 gene. The protein encoded by this gene is a DNA N-glycosylase of the endonuclease III family. Like a similar protein in E. coli, the encoded protein has DNA glycosylase activity on DNA substrates containing oxidized pyrimidine residues and has apurinic/apyrimidinic lyase activity.

Molecular Weight: 34 kDa

Gene ID: 4913

UniProt: P78549