NT5DC3 antibody (AA 52-548)

Catalog No. ABIN7601959

Product Details anti-NT5DC3 Antibody

Target: NT5DC3 (5'-Nucleotidase Domain Containing 3 (NT5DC3))

Binding Specificity: AA 52-548

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NT5DC3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Purpose: Anti-NT5DC3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NT5DC3 Antibody Picoband® (ABIN7601959). Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NT5DC3 recombinant protein (Position: D52-K548).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. van den Akker, E., Vankan-Berkhoudt, Y., Valk, P. J. M., Lowenberg, B., Delwel, R. The common viral insertion site Evi12 is located in the 5-prime-noncoding region of Gnn, a novel gene with enhanced expression in two subclasses of human acute myeloid leukemia. J. Virol. 79: 5249-5258, 2005. 2. Yatsuoka, T., Furukawa, T., Sunamura, M., Matsuno, S., Horii, A. TU12B1-TY, a novel gene in the region at 12q22-q23.1 frequently deleted in pancreatic cancer, shows reduced expression in pancreatic cancer cells. Oncol. Rep. 12: 1263-1268, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NT5DC3

Target: NT5DC3 (5'-Nucleotidase Domain Containing 3 (NT5DC3))

Alternative Name: NT5DC3 (NT5DC3 Products)

Background:

Synonyms: Kelch repeat and BTB domain-containing protein 2, BTB and kelch domain-containing protein 1, KBTBD2, BKLHD1, KIAA1489

Tissue Specificity: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Background: 5'-Nucleotidase domain containing 2 (NT5DC2) is a member of the NT5DC family and contains a haloacid dehalogenase motif localized in the N-terminus of these proteins. NT5DC2 shows high sequence similarity with NT5C2. NT5C2 has received attention in the field of hematological neoplasms because NT5C2 mutations have been demonstrated to drive resistance to thiopurine, a drug frequently used to treat hematological neoplasms. NT5C2 catalyzes purine-nucleotide metabolism and induces chemotherapeutic resistance via excess export of purines to the extracellular space and depletion of the intracellular purine-nucleotide pool. Therefore, NT5DC2 may also participate in purine-nucleotide metabolism and exert purine-nucleotide catalytic activity. NT5DC2 overexpression promotes HCC cell proliferation and clone formation by regulating the cell cycle and promoting tumor growth in subcutaneous xenografts, while NT5DC2 knockdown inhibits these processes. NT5DC2 is associated with attention-deficit/hyperactivity disorder and bipolar disorder. NT5DC2 interacts with tyrosine hydroxylase (TH) to regulate TH catalytic activity and thus regulate catecholamine synthesis. NT5DC2 has been shown to interact with and stabilize Fyn, an Src family proto-oncogene, and plays a role in regulating glioblastoma progression.

Molecular Weight: 55 kDa

Gene ID: 51559