ENO3 antibody (AA 53-245)

Catalog No. ABIN7601973

This Rabbit Polyclonal antibody specifically detects ENO3 in WB, ELISA, ICC, IF and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ENO3 antibody (AA 53-245) (ABIN7601973)

Target: ENO3 (Enolase 3 (Beta, Muscle) (ENO3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENO3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-ENO3 Antibody

Binding Specificity: AA 53-245

Purpose: Anti-ENO3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ENO3 Antibody Picoband® (ABIN7601973). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ENO3 recombinant protein (Position: D53-D245).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chen, S.-H., Giblett, E. R. Enolase: human tissue distribution and evidence for three different loci. Ann. Hum. Genet. 39: 277-280, 1976. 2. Comi, G. P., Fortunato, F., Lucchiari, S., Bordoni, A., Prelle, A., Jann, S., Keller, A., Ciscato, P., Galbiati, S., Chiveri, L., Torrente, Y., Scarlato, G., Bresolin, N. Beta-enolase deficiency, a new metabolic myopathy of distal glycolysis. Ann. Neurol. 50: 202-207, 2001. 3. Craig, S. P., Day, I. N. M., Thompson, R. J., Craig, I. W. Localization of human neurone-specific enolase to chromosome 12p13. (Abstract) Cytogenet. Cell Genet. 51: 980 only, 1989.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ENO3

Target: ENO3 (Enolase 3 (Beta, Muscle) (ENO3))

Alternative Name: ENO3

Background:

Synonyms: Intraflagellar transport protein 88 homolog, Recessive polycystic kidney disease protein Tg737 homolog, Tetratricopeptide repeat protein 10, TPR repeat protein 10, IFT88, TG737, TTC10

Tissue Specificity: Expressed in the heart, brain, liver, lung, kidney, skeletal muscle and pancreas.

Background: Enolase 3 (ENO3), more commonly known as beta-enolase (ENO-β), is an enzyme that in humans is encoded by the ENO3 gene. This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.

Molecular Weight: 47 kDa

Gene ID: 2027

UniProt: P13929