Adhesion G Protein-Coupled Receptor G1 (ADGRG1) (AA 53-554) antibody

Catalog No. ABIN7601979

This Rabbit Polyclonal antibody specifically detects in ELISA, FACS, WB and IHC. It exhibits reactivity toward Rat and Mouse.

Quick Overview for Adhesion G Protein-Coupled Receptor G1 (ADGRG1) (AA 53-554) antibody (ABIN7601979)

Target: Adhesion G Protein-Coupled Receptor G1 (ADGRG1)

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)

Product Details

Binding Specificity: AA 53-554

Purpose: Anti-Adgrg1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Adgrg1 Antibody Picoband® (ABIN7601979). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Adgrg1 recombinant protein (Position: E53-S554).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Bae, B.-I., Tietjen, I., Atabay, K. D., Evrony, G. D., Johnson, M. B., Asare, E., Wang, P. P., Murayama, A. Y., Im, K., Lisgo, S. N., Overman, L., Sestan, N., Chang, B. S., Barkovich, A. J., Grant, P. E., Topcu, M., Politsky, J., Okano, H., Piao, X., Walsh, C. A. Evolutionarily dynamic alternative splicing of GPR56 regulates regional cerebral cortical patterning. Science 343: 764-768, 2014. 2. Chiang, N.-Y., Hsiao, C.-C., Huang, Y.-S., Chen, H.-Y., Hsieh, I.-J., Chang, G.-W., Lin, H.-H. Disease-associated GPR56 mutations cause bilateral frontoparietal polymicrogyria via multiple mechanisms. J. Biol. Chem. 286: 14215-14225, 2011. 3. Kim, J.-E., Han, J. M., Park, C. R., Shin, K.-J., Ahn, C., Seong, J. Y., Hwang, J.-I. Splicing variants of the orphan G-protein-coupled receptor GPR56 regulate the activity of transcription factors associated with tumorigenesis. J. Cancer Res. Clin. Oncol. 136: 47-53, 2010.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details

Target: Adhesion G Protein-Coupled Receptor G1 (ADGRG1)

Alternative Name: Adgrg1

Background:

Synonyms: Phosphopantothenate--cysteine ligase, Phosphopantothenoylcysteine synthetase, PPC synthetase, PPCS, COAB

Tissue Specificity: Expressed in heart, placenta, skeletal muscle kidney, pancreas, spleen, prostate, testis, ovary, ileum and colon. Expressed in lung endothelial and smooth muscle cells (at protein level).

Background: Enables several functions, including collagen binding activity, extracellular matrix binding activity, and heparin binding activity. Involved in several processes, including negative regulation of neuron migration, positive regulation of Rho protein signal transduction, and seminiferous tubule development. Acts upstream of or within cerebral cortex regionalization, hematopoietic stem cell homeostasis, and positive regulation of neural precursor cell proliferation. Located in glial limiting end-foot. Is expressed in several structures, including alimentary system, ganglia, genitourinary system, respiratory system, and sensory organ. Human ortholog(s) of this gene implicated in bilateral frontoparietal polymicrogyria and bilateral perisylvian polymicrogyria. Orthologous to human ADGRG1 (adhesion G protein-coupled receptor G1).

Molecular Weight: 78 kDa

Gene ID: 14766

UniProt: Q8K209