ARHGEF6 antibody (AA 54-765)

Catalog No. ABIN7602002

This anti-ARHGEF6 antibody is a Rabbit Polyclonal antibody detecting ARHGEF6 in ELISA, WB and FACS. Suitable for Human and Mouse.

Quick Overview for ARHGEF6 antibody (AA 54-765) (ABIN7602002)

Target: ARHGEF6 (rho Guanine Nucleotide Exchange Factor (GEF) 6 (ARHGEF6))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGEF6 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-ARHGEF6 Antibody

Binding Specificity: AA 54-765

Purpose: Anti-ARHGEF6 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-ARHGEF6 Antibody Picoband® (ABIN7602002). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ARHGEF6 recombinant protein (Position: E54-R765). Human ARHGEF6 shares 91.2% and 91.3% amino acid (aa) sequence identity with mouse and rat ARHGEF6, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gallo, G., Letourneau, P. C. Axon guidance: GTPases help axons reach their targets. Curr. Biol. 8: R80-R82, 1998. 2. Gedeon, A. K., Glass, I. A., Connor, J. M., Mulley, J. C. Genetic localisation of MRX27 to Xq24-26 defines another discrete gene for non-specific X-linked mental retardation. Am. J. Med. Genet. 64: 121-124, 1996. 3. Hamosh, A. Personal Communication. Baltimore, Md. 11/21/2018.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ARHGEF6

Target: ARHGEF6 (rho Guanine Nucleotide Exchange Factor (GEF) 6 (ARHGEF6))

Alternative Name: ARHGEF6

Background:

Synonyms: ARHGEF6, COOL2, KIAA0006, PIXA, Rho guanine nucleotide exchange factor 6, Alpha-Pix, COOL-2, PAK-interacting exchange factor alpha, Rac/Cdc42 guanine nucleotide exchange factor 6

Background: Rho guanine nucleotide exchange factor 6 is a protein that, in humans, is encoded by the ARHGEF6 gene. Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It may form a complex with G proteins and stimulate Rho-dependent signals. This protein is activated by PI3-kinase. Mutations in this gene can cause X-chromosomal non-specific cognitive disability.

Molecular Weight: 87 kDa

Gene ID: 9459

UniProt: Q15052

Pathways: Neurotrophin Signaling Pathway