IWS1 antibody (AA 547-771)

Catalog No. ABIN7602008

Product Details anti-IWS1 Antibody

Target: IWS1 (IWS1 Homolog (IWS1))

Binding Specificity: AA 547-771

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IWS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Purpose: Anti-IWS1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-IWS1 Antibody Picoband® (ABIN7602008). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human IWS1 recombinant protein (Position: R547-E771).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Yoh, S. M. , Cho, H. , Pickle, L. , Evans, R. M. , & Jones, K. A. . (2007). The spt6 sh2 domain binds ser2-p rnapii to iws1-dependent mrna splicing and export. Genes & Development, 21(2), 160. 2. Yoh, S. M. , Lucas, J. S. , & Jones, K. A. . (2008). The iws1:spt6:ctd complex controls cotranscriptional mrna biosynthesis and hypb/setd2-mediated histone h3k36 methylation. Genes Dev, 22(24), 3422-3434.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for IWS1

Target: IWS1 (IWS1 Homolog (IWS1))

Alternative Name: IWS1 (IWS1 Products)

Background:

Synonyms: Long-chain-fatty-acid--CoA ligase 3,6.2.1.3,Long-chain acyl-CoA synthetase 3,LACS 3,ACSL3,ACS3, FACL3, LACS3,

Tissue Specificity: Expressed in breast, ductal and invasive ductal carcinomas of the breast, sporadic colorectal adenomas and carcinomas (at protein level). Expressed in fetal brain. Expressed in lung, amygdala, eye, prostate, pancreatic and prostate cancers, head and neck tumors and embryonal tumor.

Background: Protein IWS1 homolog also known as interacts with Spt6 (IWS1) is a protein that in humans is encoded by the IWS1 gene. Various steps in gene expression, such as mRNA processing, surveillance, export, and synthesis are coupled to transcription elongation). The C-terminal domain (CTD) of the large subunit of RNA polymerase II plays an important role in the integration of these different steps. IWS1 interacts with Spt6, a CTD-binding transcription elongation factor and H3 chaperone. IWS1 also recruits another CTD-binding protein, HYPB/Setd2 histone methyltransferase, to the RNA polymerase II complex for elongation-coupled H3K36 trimethylation. Thus, IWS1 links Spt6 and HYPB/Setd2 in a large complex and regulates mRNA synthesis and histone methylation at the co-transcriptional level .

Molecular Weight: 140 kDa

Gene ID: 55677