IWS1 antibody (AA 1-50)

Catalog No. ABIN7449756

Product Details anti-IWS1 Antibody

Target: IWS1 (IWS1 Homolog (IWS1))

Binding Specificity: AA 1-50

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IWS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Purpose: Rabbit anti-IWS1 Antibody, Affinity Purified

Predicted Reactivity: Rat

Purification: Affinity Purified

Immunogen: Between AA 1 and 50

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 10 μg/mg lysate

WB: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for IWS1

Target: IWS1 (IWS1 Homolog (IWS1))

Alternative Name: IWS1 (IWS1 Products)

Synonyms: 1700069O15Rik antibody, 3000008H23 antibody, AL117927 antibody, AW214353 antibody, RGD1304762 antibody, IWS1, SUPT6H interacting protein antibody, IWS1, SUPT6 interacting protein antibody, IWS1 antibody, Iws1 antibody

Background: Background: IWS1 homolog is a transcription factor which plays a key role in defining the composition of the RNA polymerase II (RNAPII) elongation complex and in modulating the production of mature mRNA transcripts. IWS1 acts as an assembly factor to recruit various factors to the RNAPII elongation complex and is recruited to the complex via binding to the transcription elongation factor SUPT6H bound to the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2) to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription [taken from the Universal Protein Resource (UniProt) Q96ST2].

Gene ID: 55677

NCBI Accession: NP_060439

UniProt: Q96ST2