DHX29 antibody (AA 56-1343)

Catalog No. ABIN7602033

Product Details anti-DHX29 Antibody

Target: DHX29 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 29 (DHX29))

Binding Specificity: AA 56-1343

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DHX29 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Purpose: Anti-DHX29 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-DHX29 Antibody Picoband® (ABIN7602033). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DHX29 recombinant protein (Position: R56-K1343).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hartz, P. A. Personal Communication. Baltimore, Md. 4/1/2009. 2. Pisareva, V. P., Pisarev, A. V., Komar, A. A., Hellen, C. U. T., Pestova, T. V. Translation initiation on mammalian mRNAs with structured 5-prime UTRs requires DExH-box protein DHX29. Cell 135: 1237-1250, 2008.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for DHX29

Target: DHX29 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 29 (DHX29))

Alternative Name: DHX29 (DHX29 Products)

Background:

Synonyms: Fascin-2, Retinal fascin, FSCN2

Tissue Specificity: Localized specifically in the outer and inner segments of the photoreceptor cells in the retina.

Background: DExH-box helicase 29 (DHX29) is a 155 kDa protein that in humans is encoded by the DHX29 gene. This gene encodes a member of the DEAH (Asp-Glu-Ala-His) subfamily of proteins, part of the DEAD (Asp-Glu-Ala-Asp) box family of RNA helicases. The encoded protein functions in translation initiation, and is specifically required for ribosomal scanning across stable mRNA secondary structures during initiation codon selection. This protein may also play a role in sensing virally derived cytosolic nucleic acids. Knockdown of this gene results in reduced protein translation and impaired proliferation of cancer cells.

Molecular Weight: 155 kDa

Gene ID: 54505

UniProt: Q7Z478