CEP250 antibody (AA 563-853)
Quick Overview for CEP250 antibody (AA 563-853) (ABIN7602053)
Target
See all CEP250 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 563-853
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Purpose
- Anti-CEP250 Antibody
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Cross-Reactivity (Details)
- No cross-reactivity with other proteins.
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Characteristics
- Anti-C Antibody (ABIN7602053). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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Purification
- Immunogen affinity purified.
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Immunogen
- E.coli-derived human CEP250 recombinant protein (Position: Q563-K853).
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Isotype
- IgG
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Application Notes
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Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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Concentration
- 500 μg/mL
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Buffer
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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Storage
- 4 °C,-20 °C
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Storage Comment
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- CEP250 (Centrosomal Protein 250kDa (CEP250))
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Alternative Name
- CEP250
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Background
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Synonyms: Gastrotropin, GT, Fatty acid-binding protein 6, Ileal lipid-binding protein, ILBP, Intestinal 15 kDa protein, I-15P, Intestinal bile acid-binding protein, I-BABP, FABP6, ILBP, ILLBP
Tissue Specificity: Isoform 1 is expressed in the jejunum, ileum, cecum and ascending colon intestine. Isoform 2 is xpressed in the gallbladder, duodenum, jejunum, ileum, cecum, ascending, transverse and descending colon, sigmoid colon and rectum. Isoform 2 is expressed in colorectal adenocarcinomas and their adjacent normal mucosa.
Background: Centrosome-associated protein CEP250 is a protein that in humans is encoded by the CEP250 gene. This gene encodes a core centrosomal protein required for centriole-centriole cohesion during interphase of the cell cycle. The encoded protein dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome. Alternative splicing results in multiple transcript variants encoding different isoforms.
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Molecular Weight
- 250 kDa
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Gene ID
- 11190
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Pathways
- M Phase, SARS-CoV-2 Protein Interactome
Target
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