GAR1 antibody (AA 58-165)

Catalog No. ABIN7602076

The Rabbit Polyclonal anti-GAR1 antibody has been validated for ELISA, WB, IHC, IF, ICC and FACS. It is suitable to detect GAR1 in samples from Human.

Quick Overview for GAR1 antibody (AA 58-165) (ABIN7602076)

Target: GAR1 (GAR1 Ribonucleoprotein Homolog (Yeast) (GAR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAR1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-GAR1 Antibody

Binding Specificity: AA 58-165

Purpose: Anti-NOLA1/GAR1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NOLA1/GAR1 Antibody Picoband® (ABIN7602076). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NOLA1/GAR1 recombinant protein (Position: F58-K165).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Dragon, F., Pogacic, V., Filipowicz, W. In vitro assembly of human H/ACA small nucleolar RNPs reveals unique features of U17 and telomerase RNAs. Molec. Cell. Biol. 20: 3037-3048, 2000. 2. Tollervey, D., Kiss, T. Function and synthesis of small nucleolar RNAs. Curr. Opin. Cell Biol. 9: 337-342, 1997.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for GAR1

Target: GAR1 (GAR1 Ribonucleoprotein Homolog (Yeast) (GAR1))

Alternative Name: GAR1

Background:

Synonyms: Forkhead box protein F1, Forkhead-related activator 1, FREAC-1, Forkhead-related protein FKHL5, Forkhead-related transcription factor 1, FOXF1, FKHL5, FREAC1

Tissue Specificity: Expressed in kidney.

Background: H/ACA ribonucleoprotein complex subunit 1 is a protein that in humans is encoded by the GAR1 gene. This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA2 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. These four H/ACA snoRNP proteins are also components of the telomerase complex. The encoded protein of this gene contains two glycine- and arginine-rich domains and is related to Saccharomyces cerevisiae Gar1p. Two splice variants have been found for this gene.

Molecular Weight: 25 kDa

Gene ID: 54433