EHMT2 antibody (AA 580-1195)

Catalog No. ABIN7602084

This Rabbit Polyclonal antibody specifically detects EHMT2 in WB, ELISA, IHC, FACS, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for EHMT2 antibody (AA 580-1195) (ABIN7602084)

Target: EHMT2 (Ankyrin Repeat-Containing Protein (EHMT2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EHMT2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-EHMT2 Antibody

Binding Specificity: AA 580-1195

Purpose: Anti-EHMT2/G9A Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-EHMT2/G9A Antibody Picoband® (ABIN7602084). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human EHMT2 recombinant protein (Position: Q580-H1195).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ding, N., Zhou, H., Esteve, P.-O., Chin, H. G., Kim, S., Xu, X., Joseph, S. M., Friez, M. J., Schwartz, C. E., Pradhan, S., Boyer, T. G. Mediator links epigenetic silencing of neuronal gene expression with X-linked mental retardation. Molec. Cell 31: 347-359, 2008. 2. Dutta, A., Le Magnen, C., Mitrofanova, A., Ouyang, X., Califano, A., Abate-Shen, C. Identification of an NKX3.1-G9a-UTY transcriptional regulatory network that controls prostate differentiation. Science 352: 1576-1580, 2016. 3. Maier, V. K., Feeney, C. M., Taylor, J. E., Creech, A. L., Qiao, J. W., Szanto, A., Das, P. P., Chevrier, N., Cifuentes-Rojas, C., Orkin, S. H., Carr, S. A., Jaffe, J. D., Mertins, P., Lee, J. T. Functional proteomic analysis of repressive histone methyltransferase complexes reveals ZNF518B as a G9A regulator. Molec. Cell. Proteomics 14: 1435-1446, 2015.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for EHMT2

Target: EHMT2 (Ankyrin Repeat-Containing Protein (EHMT2))

Alternative Name: EHMT2

Background:

Synonyms: Neuronal migration protein doublecortin,Doublin,Lissencephalin-X,Lis-X,DCX,DBCN, LISX,

Tissue Specificity: Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.

Background: Euchromatic histone-lysine N-methyltransferase 2 (EHMT2), also known as G9a, is a histone methyltransferase enzyme that in humans is encoded by the EHMT2 gene. This gene encodes a methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 by this protein results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA locus. Alternative splicing results in multiple transcript variants.

Molecular Weight: 160-180 kDa

Gene ID: 10919