Calpain 5 antibody (AA 583-616)

Catalog No. ABIN7602087

This Rabbit Polyclonal antibody specifically detects Calpain 5 in WB, IF, ELISA, ICC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Calpain 5 antibody (AA 583-616) (ABIN7602087)

Target: Calpain 5 (CAPN5)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calpain 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-Calpain 5 Antibody

Binding Specificity: AA 583-616

Purpose: Anti-CAPN5 Antibody Picoband®

Characteristics: Anti-CAPN5 Antibody Picoband® (ABIN7602087). Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CAPN5 recombinant protein (Position: N583-N616). Human CAPN5 shares 84.8% and 78.8% amino acid (aa) sequence identity with mouse and rat CAPN5, respectively.

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bennett, S. R., Folk, J. C., Kimura, A. E., Russell, S. R., Stone, E. M., Raphtis, E. M. Autosomal dominant neovascular inflammatory vitreoretinopathy. Ophthalmology 97: 1125-1135, 1990. 2. Dear, N., Matena, K., Vingron, M., Boehm, T. A new subfamily of vertebrate calpains lacking a calmodulin-like domain: implications for calpain regulation and evolution. Genomics 45: 175-184, 1997. 3. Franz, T., Winckler, L., Boehm, T., Dear, T. N. Capn5 is expressed in a subset of T cells and is dispensable for development. Molec. Cell. Biol. 24: 1649-1654, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Calpain 5

Target: Calpain 5 (CAPN5)

Alternative Name: CAPN5

Background: Calpain-5 is a protein that in humans is encoded by the CAPN5 gene. Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3.

Molecular Weight: 73 kDa

Gene ID: 726

UniProt: O15484