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Calpain 5 antibody

CAPN5 Reactivity: Human WB, IF, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2855597
  • Target See all Calpain 5 (CAPN5) Antibodies
    Calpain 5 (CAPN5)
    Reactivity
    • 26
    • 15
    • 14
    Human
    Host
    • 25
    • 4
    Rabbit
    Clonality
    • 26
    • 3
    Polyclonal
    Conjugate
    • 21
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Calpain 5 antibody is un-conjugated
    Application
    • 17
    • 7
    • 6
    • 6
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Cross-Reactivity
    Human, Mouse
    Characteristics
    Rabbit polyclonal antibody to Calpain-5 (calpain 5)
    Calpain-5 antibody [N1C1]
    Purification
    Purified by antigen-affinity chromatography.
    Immunogen
    Recombinant protein encompassing a sequence within the center region of human Calpain 5. The exact sequence is proprietary.
    Isotype
    IgG
  • Application Notes
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
    Comment

    Positive Control: mouse uterus , RMS-13

    Validation: Orthogonal, Overexpression

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.39 mg/mL
    Buffer
    1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Velez, Bassuk, Schaefer, Brooks, Gakhar, Mahajan, Kahn, Tsang, Ferguson, Mahajan: "A novel de novo CAPN5 mutation in a patient with inflammatory vitreoretinopathy, hearing loss, and developmental delay." in: Cold Spring Harbor molecular case studies, Vol. 4, Issue 3, (2019) (PubMed).

    Schaefer, Toral, Velez, Cox, Baker, Borcherding, Colgan, Bondada, Mashburn, Yu, Geddes, Tsang, Bassuk, Mahajan: "Calpain-5 Expression in the Retina Localizes to Photoreceptor Synapses." in: Investigative ophthalmology & visual science, Vol. 57, Issue 6, pp. 2509-21, (2016) (PubMed).

  • Target
    Calpain 5 (CAPN5)
    Alternative Name
    calpain 5 (CAPN5 Products)
    Synonyms
    HTRA3 antibody, nCL-3 antibody, Htra3 antibody, capn5 antibody, MGC53448 antibody, MGC89353 antibody, CAPN5 antibody, zgc:153446 antibody, calpain 5 antibody, calpain 5 L homeolog antibody, calpain 5a antibody, calpain 6 antibody, CAPN5 antibody, Capn5 antibody, capn5.L antibody, capn5 antibody, capn5a antibody, Bm1_30735 antibody, Bm1_30740 antibody, CAPN6 antibody, LOAG_10085 antibody
    Background
    Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3.

    Molecular Weight
    73 kDa
    Gene ID
    726
    UniProt
    O15484
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