PRSS8 antibody (AA 59-322)

Catalog No. ABIN7602095

This anti-PRSS8 antibody is a Rabbit Polyclonal antibody detecting PRSS8 in WB, ELISA and FACS. Suitable for Human.

Quick Overview for PRSS8 antibody (AA 59-322) (ABIN7602095)

Target: PRSS8 (Protease, serine, 8 (PRSS8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRSS8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-PRSS8 Antibody

Binding Specificity: AA 59-322

Purpose: Anti-PRSS8 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PRSS8 Antibody Picoband® (ABIN7602095). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PRSS8 recombinant protein (Position: Q59-R322).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Donaldson, S. H., Hirsh, A., Li, D. C., Holloway, G., Chao, J., Boucher, R. C., Gabriel, S. E. Regulation of the epithelial sodium channel by serine proteases in human airways. J. Biol. Chem. 277: 8338-8345, 2002. 2. Yu, J. X., Chao, L., Chao, J. Prostasin is a novel human serine proteinase from seminal fluid: purification, tissue distribution, and localization in prostate gland. J. Biol. Chem. 269: 18843-18848, 1994. 3. Yu, J. X., Chao, L., Chao, J. Molecular cloning, tissue-specific expression, and cellular localization of human prostasin mRNA. J. Biol. Chem. 270: 13483-13489, 1995.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PRSS8

Target: PRSS8 (Protease, serine, 8 (PRSS8))

Alternative Name: PRSS8

Background:

Synonyms: Dynein light chain Tctex-type 1, Protein CW-1, T-complex testis-specific protein 1 homolog, DYNLT1, TCTEL1, TCTEX-1, TCTEX1

Tissue Specificity: Expressed in heart, placenta, skeletal muscle kidney, pancreas, spleen, prostate, testis, ovary, ileum and colon. Expressed in lung endothelial and smooth muscle cells (at protein level).

Background: Prostasin is a protein that in humans is encoded by the PRSS8 gene This gene encodes a member of the peptidase S1 or chymotrypsin family of serine proteases. The encoded preproprotein is proteolytically processed to generate light and heavy chains that associate via a disulfide bond to form the heterodimeric enzyme. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. This protease exhibits trypsin-like substrate specificity, cleaving protein substrates at the carboxyl terminus of lysine or arginine residues. The encoded protease partially mediates proteolytic activation of the epithelial sodium channel, a regulator of sodium balance, and may also play a role in epithelial barrier formation.

Molecular Weight: 38 kDa

Gene ID: 5652

UniProt: Q16651