Nicalin antibody (AA 59-563)

Catalog No. ABIN7602104

This anti-Nicalin antibody is a Rabbit Polyclonal antibody detecting Nicalin in WB, ELISA, IHC, IF, ICC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for Nicalin antibody (AA 59-563) (ABIN7602104)

Target: Nicalin (NCLN)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nicalin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-Nicalin Antibody

Binding Specificity: AA 59-563

Purpose: Anti-NCLN Antibody Picoband®

Characteristics: Anti-NCLN Antibody Picoband® (ABIN7602104). Tested in WB, IHC, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NCLN recombinant protein (Position: Q59-Q563). Human NCLN shares 92.9% and 92.5% amino acid (aa) sequence identity with mouse and rat NCLN, respectively.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Dettmer, U., Kuhn, P.-H., Abou-Ajram, C., Lichtenthaler, S. F., Kruger, M., Kremmer, E., Haass, C., Haffner, C. Transmembrane protein 147 (TMEM147) is a novel component of the nicalin-NOMO protein complex. J. Biol. Chem. 285: 26174-26181, 2010. 2. Haffner, C., Frauli, M., Topp, S., Irmler, M., Hofmann, K., Regula, J. T., Bally-Cuif, L., Haass, C. Nicalin and its binding partner Nomo are novel Nodal signaling antagonists. EMBO J. 23: 3041-3050, 2004. 3. Stumpf, A. M. Personal Communication. Baltimore, Md. 08/17/2021.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Nicalin

Target: Nicalin (NCLN)

Alternative Name: NCLN

Background: Nicalin is a protein that in humans is encoded by the NCLN gene. Enables ribosome binding activity. Involved in protein stabilization, regulation of protein complex stability, and regulation of protein-containing complex assembly. Located in endoplasmic reticulum membrane. Part of protein-containing complex.

Molecular Weight: 63 kDa

Gene ID: 56926

UniProt: Q969V3