Nicalin antibody (AA 59-563)
Quick Overview for Nicalin antibody (AA 59-563) (ABIN7602104)
Target
See all Nicalin (NCLN) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 59-563
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Purpose
- Anti-NCLN Antibody
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Characteristics
- Anti-NCLN Antibody (ABIN7602104). Tested in WB, IHC, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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Purification
- Immunogen affinity purified.
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Immunogen
- E.coli-derived human NCLN recombinant protein (Position: Q59-Q563). Human NCLN shares 92.9% and 92.5% amino acid (aa) sequence identity with mouse and rat NCLN, respectively.
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Isotype
- IgG
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Application Notes
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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Concentration
- 500 μg/mL
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Buffer
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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Storage
- 4 °C,-20 °C
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Storage Comment
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- Nicalin (NCLN)
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Alternative Name
- NCLN
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Background
- Nicalin is a protein that in humans is encoded by the NCLN gene. Enables ribosome binding activity. Involved in protein stabilization, regulation of protein complex stability, and regulation of protein-containing complex assembly. Located in endoplasmic reticulum membrane. Part of protein-containing complex.
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Molecular Weight
- 63 kDa
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Gene ID
- 56926
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UniProt
- Q969V3
Target
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