Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 594-1909) antibody

Catalog No. ABIN7602111

This Rabbit Polyclonal antibody specifically detects in WB, FACS, IHC and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 594-1909) antibody (ABIN7602111)

Target: Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA

Product Details

Binding Specificity: AA 594-1909

Purpose: Anti-SPTBN2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SPTBN2 Antibody Picoband® (ABIN7602111). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SPTBN2 recombinant protein (Position: A594-K1909).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Al-Muhaizea, M. A., AlMutairi, F., Almass, R., AlHarthi, S., Aldosary, M. S., Alsagob, M., AlOdaib, A., Colak, D., Kaya, N. A novel homozygous mutation in SPTBN2 leads to spinocerebellar ataxia in a consanguineous family: report of a new infantile-onset case and brief review of the literature. Cerebellum 17: 276-285, 2018. 2. Clarkson, Y. L., Gillespie, T., Perkins, E. M., Lyndon, A. R., Jackson, M. Beta-III spectrin mutation L253P associated with spinocerebellar ataxia type 5 interferes with binding to Arp1 and protein trafficking from the Golgi. Hum. Molec. Genet. 19: 3634-3641, 2010. 3. Clarkson, Y. L., Perkins, E. M., Cairncross, C. J., Lyndon, A. R., Skehel, P. A., Jackson, M. Beta-III spectrin underpins ankyrin R function in Purkinje cell dendritic trees: protein complex critical for sodium channel activity is impaired by SCA5-associated mutations. Hum. Molec. Genet. 23: 3875-3882, 2014.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details

Target: Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)

Alternative Name: SPTBN2

Background:

Synonyms: Atypical chemokine receptor 2, C-C chemokine receptor D6, Chemokine receptor CCR-10, Chemokine receptor CCR-9, Chemokine-binding protein 2, Chemokine-binding protein D6, ACKR2, CCBP2, CCR10, CMKBR9, D6

Tissue Specificity: Found in endothelial cells lining afferent lymphatics in dermis and lymph nodes. Also found in lymph nodes subcapsular and medullary sinuses, tonsillar lymphatic sinuses and lymphatics in mucosa and submucosa of small and large intestine and appendix. Also found in some malignant vascular tumors. Expressed at high levels in Kaposi sarcoma-related pathologies. Expressed on apoptotic neutrophils (at protein level). Expressed primarily in placenta and fetal liver, and found at very low levels in the lung and lymph node.

Background: Spectrin beta chain, brain 2 is a protein that in humans is encoded by the SPTBN2 gene. Spectrins are principle components of a cell's membrane-cytoskeleton and are composed of two alpha and two beta spectrin subunits. The protein encoded by this gene (SPTBN2), is called spectrin beta non-erythrocytic 2 or beta-III spectrin. It is related to, but distinct from, the beta-II spectrin gene which is also known as spectrin beta non-erythrocytic 1 (SPTBN1). SPTBN2 regulates the glutamate signaling pathway by stabilizing the glutamate transporter EAAT4 at the surface of the plasma membrane. Mutations in this gene cause a form of spinocerebellar ataxia, SCA5, that is characterized by neurodegeneration, progressive locomotor incoordination, dysarthria, and uncoordinated eye movements.

Molecular Weight: 271 kDa

Gene ID: 6712

UniProt: O15020

Pathways: Regulation of Actin Filament Polymerization, Synaptic Vesicle Exocytosis