ALDH1A1 antibody (AA 6-342)

Catalog No. ABIN7602127

The Rabbit Polyclonal anti-ALDH1A1 antibody has been validated for WB, IHC, ELISA, IF, FACS and ICC. It is suitable to detect ALDH1A1 in samples from Human, Mouse and Rat.

Quick Overview for ALDH1A1 antibody (AA 6-342) (ABIN7602127)

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALDH1A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-ALDH1A1 Antibody

Binding Specificity: AA 6-342

Purpose: Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody (ABIN7602127). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ALDH1A1 recombinant protein (Position: T6-Q342).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Eckey, R., Agarwal, D. P., Saha, N., Goedde, H. W. Detection and partial characterization of a variant form of cytosolic aldehyde dehydrogenase isozyme. Hum. Genet. 72: 95-97, 1986. 2. Hempel, J., von Bahr-Lindstrom, H., Jornvall, H. Aldehyde dehydrogenase from human liver: primary structure of the cytoplasmic isoenzyme. Europ. J. Biochem. 141: 21-35, 1984.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ALDH1A1

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Alternative Name: ALDH1A1

Background:

Synonyms: Retinal dehydrogenase 1, RALDH 1, RalDH1, ALDH-E1, ALHDII, Aldehyde dehydrogenase family 1 member A, Aldehyde dehydrogenase, cytosolic, ALDH1A1, ALDC, ALDH1

Tissue Specificity: Strong expression found in normal gastric mucosa. Not detected in the small or large intestine nor in normal mammary gland.

Background: Aldehyde dehydrogenase 1 family, member A1, also known as ALDH1A1 or retinaldehyde dehydrogenase 1 (RALDH1), is an enzyme that in humans is encoded by the ALDH1A1 gene. It is mapped to 9q21.13. The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet.

Molecular Weight: 55 kDa

Gene ID: 216

UniProt: P00352

Pathways: Dopaminergic Neurogenesis