POC1A antibody (AA 6-371)

Catalog No. ABIN7602131

Product Details anti-POC1A Antibody

Target: POC1A (POC1 Centriolar Protein Homolog A (POC1A))

Binding Specificity: AA 6-371

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POC1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purpose: Anti-POC1A Antibody Picoband®

Characteristics: Anti-POC1A Antibody Picoband® (ABIN7602131). Tested in WB, IHC, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human POC1A recombinant protein (Position: A6-D371). Human POC1A shares 91% amino acid (aa) sequence identity with mouse POC1A.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hames, R. S., Hames, R., Prosser, S. L., Euteneuer, U., Lopes, C. A. M., Moore, W., Woodland, H. R., Fry, A. M. Pix1 and Pix2 are novel WD40 microtubule-associated proteins that colocalize with mitochondria in Xenopus germ plasm and centrosomes in human cells. Exp. Cell Res. 314: 574-589, 2008. 2. Keller, L. C., Geimer, S., Romijn, E., Yates, J., III, Zamora, I., Marshall, W. F. Molecular architecture of the centriole proteome: the conserved WD40 domain protein POC1 is required for centriole duplication and length control. Molec. Biol. Cell 20: 1150-1166, 2009. 3. Sarig, O., Nahum, S., Rapaport, D., Ishida-Yamamoto, A., Fuchs-Telem, D., Qiaoli, L., Cohen-Katsenelson, K., Spiegel, R., Nousbeck, J., Israeli, S., Borochowitz, Z.-U., Padalon-Brauch, G., Uitto, J., Horowitz, M., Shalev, S., Sprecher, E. Short stature, onychodysplasia, facial dysmorphism, and hypotrichosis syndrome is caused by a POC1A mutation. Am. J. Hum. Genet. 91: 337-342, 2012.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for POC1A

Target: POC1A (POC1 Centriolar Protein Homolog A (POC1A))

Alternative Name: POC1A (POC1A Products)

Background: POC1 proteins contain an N-terminal WD40 domain and a C-terminal coiled coil domain and are part of centrosomes. They play an important role in basal body and cilia formation. This gene encodes one of the two POC1 proteins found in humans. Mutations in this gene result in short stature, onychodysplasia, facial dysmorphism, and hypotrichosis (SOFT) syndrome.

Molecular Weight: 40 kDa

Gene ID: 25886