TXN2 antibody (AA 60-166)

Catalog No. ABIN7602148

The Mouse Monoclonal anti-TXN2 antibody has been validated for WB, IHC, IF, ICC and FACS. It is suitable to detect TXN2 in samples from Human, Rat and Mouse.

Quick Overview for TXN2 antibody (AA 60-166) (ABIN7602148)

Target: TXN2 (Thioredoxin 2 (TXN2))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This TXN2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 4H3

Product Details anti-TXN2 Antibody

Binding Specificity: AA 60-166

Purpose: Anti-Thioredoxin 2/TXN2 Antibody Picoband® (monoclonal, 4H3)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Thioredoxin 2/TXN2 Antibody Picoband® (monoclonal, 4H3) (ABIN7602148). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Thioredoxin 2/TXN2 recombinant protein (Position: T60-G166).

Isotype: IgG2a

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunofluorescence, 2 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Benhar, M., Forrester, M. T., Hess, D. T., Stamler, J. S. Regulated protein denitrosylation by cytosolic and mitochondrial thioredoxins. Science 320: 1050-1054, 2008. 2. Chen, Y., Cai, J., Murphy, T. J., Jones, D. P. Overexpressed human mitochondrial thioredoxin confers resistance to oxidant-induced apoptosis in human osteosarcoma cells. J. Biol. Chem. 277: 33242-33248, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for TXN2

Target: TXN2 (Thioredoxin 2 (TXN2))

Alternative Name: TXN2

Background:

Synonyms: Thioredoxin, mitochondrial, MTRX, Mt-Trx, Thioredoxin-2, TXN2, TRX2

Tissue Specificity: Widely expressed in adult (at protein level) and fetal tissues.

Background: Thioredoxin, mitochondrial also known as thioredoxin-2 is a protein that in humans is encoded by the TXN2 gene on chromosome 22. It is mapped to 22q12.3. This nuclear gene encodes a mitochondrial member of the thioredoxin family, a group of small multifunctional redox-active proteins. The encoded protein may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis.

Molecular Weight: 14 kDa

Gene ID: 25828

UniProt: Q99757

Pathways: Cell RedoxHomeostasis