POLR1A antibody (AA 607-1720)

Catalog No. ABIN7602161

This anti-POLR1A antibody is a Rabbit Polyclonal antibody detecting POLR1A in WB and ELISA. Suitable for Human.

Quick Overview for POLR1A antibody (AA 607-1720) (ABIN7602161)

Target: POLR1A (Polymerase (RNA) I Polypeptide A, 194kDa (POLR1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-POLR1A Antibody

Binding Specificity: AA 607-1720

Purpose: Anti-POLR1A Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-POLR1A Antibody Picoband® (ABIN7602161). Tested in WB, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human POLR1A recombinant protein (Position: E607-R1720). Human POLR1A shares 86.3% and 87.3% amino acid (aa) sequence identity with mouse and rat POLR1A, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL
1. da Rocha, L. A., Pires, L. V. L., Yamamoto, G. L., Magliocco Ceroni, J. R., Honjo, R. S., de Novaes Franca Bisneto, E., Oliveira, L. A. N., Rosenberg, C., Krepischi, A. C. V., Passos-Bueno, M. R., Kim, C. A., Bertola, D. R. Congenital limb deficiency: genetic investigation of 44 individuals presenting mainly longitudinal defects in isolated or syndromic forms. Clin. Genet. 100: 615-623, 2021. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 6/2/2015. 3. Kara, B., Koroglu, C., Peltonen, K., Steinberg, R., Maras Genc, H., Holtta-Vuori, M., Guven, A., Kanerva, K., Kotil, T., Solakoglu, S., Zhou, Y., Olkkonen, V. M., Ikonen, E., Laiho, M., Tolun, A. Severe neurodegenerative disease in brothers with homozygous mutation in POLR1A. Europ. J. Hum. Genet. 25: 315-323, 2017.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for POLR1A

Target: POLR1A (Polymerase (RNA) I Polypeptide A, 194kDa (POLR1A))

Alternative Name: POLR1A

Background:

Synonyms: POLR1A, DNA-directed RNA polymerase I subunit RPA1, RNA polymerase I subunit A1, EC 2.7.7.6, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194 kDa subunit, RPA194

Background: DNA-directed RNA polymerase I subunit RPA1 is an enzyme that in humans is encoded by the POLR1A gene. The protein encoded by this gene is the largest subunit of the RNA polymerase I complex. The encoded protein represents the catalytic subunit of the complex, which transcribes DNA into ribosomal RNA precursors. Defects in this gene are a cause of the Cincinnati type of acrofacial dysostosis.

Molecular Weight: 195 kDa

Gene ID: 25885

UniProt: O95602