CDH15 antibody (AA 61-742)

Catalog No. ABIN7602173

This anti-CDH15 antibody is a Rabbit Polyclonal antibody detecting CDH15 in WB, ELISA, IHC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for CDH15 antibody (AA 61-742) (ABIN7602173)

Target: CDH15 (M-Cadherin (CDH15))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDH15 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-CDH15 Antibody

Binding Specificity: AA 61-742

Purpose: Anti-M Cadherin/CDH15 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-M Cadherin/CDH15 Antibody Picoband® (ABIN7602173). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human M Cadherin/CDH15 recombinant protein (Position: L61-D742).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Bhalla, K., Luo, Y., Buchan, T., Beachem, M. A., Guzauskas, G. F., Ladd, S., Bratcher, S. J., Schroer, R. J., Balsamo, J., DuPont, B. R., Lilien, J., Srivastava, A. K. Alterations in CDH15 and KIRREL3 in patients with mild to severe intellectual disability. Am. J. Hum. Genet. 83: 703-713, 2008. 2. Donalies, M., Cramer, M., Ringwald, M., Starzinski-Powitz, A. Expression of M-cadherin, a member of the cadherin multigene family, correlates with differentiation of skeletal muscle cells. Proc. Nat. Acad. Sci. 88: 8024-8028, 1991. 3. Hollnagel, A., Grund, C., Franke, W. W., Arnold, H.-H. The cell adhesion molecule M-cadherin is not essential for muscle development and regeneration. Molec. Cell. Biol. 22: 4760-4770, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CDH15

Target: CDH15 (M-Cadherin (CDH15))

Alternative Name: CDH15

Background:

Synonyms: Interleukin-17B, IL-17B, Cytokine CX1, Cytokine-like protein ZCYTO7, Neuronal interleukin-17-related factor, Il17b, Nirf, Zcyto7

Tissue Specificity: Expressed in adult pancreas, small intestine, stomach, spinal cord and testis. Less pronounced expression in prostate, colon mucosal lining, and ovary.

Background: Cadherin-15 is a protein that in humans is encoded by the CDH15 gene. This gene is a member of the cadherin superfamily of genes, encoding calcium-dependent intercellular adhesion glycoproteins. Cadherins consist of an extracellular domain containing 5 cadherin domains, a transmembrane region, and a conserved cytoplasmic domain. Transcripts from this particular cadherin are expressed in myoblasts and upregulated in myotubule-forming cells. The protein is thought to be essential for the control of morphogenetic processes, specifically myogenesis, and may provide a trigger for terminal muscle cell differentiation.

Molecular Weight: 130 kDa

Gene ID: 1013

UniProt: P55291