SH3G2 antibody (AA 65-292)

Catalog No. ABIN7602229

The Rabbit Polyclonal anti-SH3G2 antibody has been validated for WB, ELISA, IHC, IF, ICC and FACS. It is suitable to detect SH3G2 in samples from Human, Mouse and Rat.

Quick Overview for SH3G2 antibody (AA 65-292) (ABIN7602229)

Target: SH3G2 (Endophilin-A1 (SH3G2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SH3G2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-SH3G2 Antibody

Binding Specificity: AA 65-292

Purpose: Anti-SH3GL2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SH3GL2 Antibody Picoband® (ABIN7602229). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SH3GL2 recombinant protein (Position: R65-Q292).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gallop, J. L., Butler, P. J. G., McMahon, H. T. Endophilin and CtBP/BARS are not acyl transferases in endocytosis or Golgi fission. Nature 438: 675-678, 2005. 2. Giachino, C., Lantelme, E., Lanzetti, L., Saccone, S., Della Valle, G., Migone, N. A novel SH3-containing human gene family preferentially expressed in the central nervous system. Genomics 41: 427-434, 1997. 3. Howard, L., Nelson, K. K., Maciewicz, R. A., Blobel, C. P. Interaction of the metalloprotease disintegrins MDC9 and MDC15 with two SH3 domain-containing proteins, endophilin I and SH3PX1. J. Biol. Chem. 274: 31693-31699, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SH3G2

Target: SH3G2 (Endophilin-A1 (SH3G2))

Alternative Name: SH3GL2

Background:

Synonyms: POU domain, class 4, transcription factor 1

Tissue Specificity: Expressed in the brain and the retina. Present in the developing brain, spinal cord and eye.

Background: Endophilin-A1 is a protein that in humans is encoded by the SH3GL2 gene. Endophilin proteins are part of a large family of Bin/Amphiphysin/Rvs (BAR) domain proteins that are involved in cell membrane remodeling. The endophilins are encoded by five genes, which produce endophilin A 1-3 and B 1-2. Endophilins are involved in many cellular mechanisms, such as synaptic vesicle recycling, receptor trafficking, and membrane remodeling processes. Research studies indicate that endophilin 1 (endophilin A1, SH3GL2) can induce different membrane shapes and participate in the morphogenesis of dendritic spines. Endophilin 1 is also involved in regulating blood brain barrier permeability via the EGFR-JNK pathway.

Molecular Weight: 41 kDa

Gene ID: 6456

UniProt: Q99962

Pathways: Neurotrophin Signaling Pathway, EGFR Downregulation