CARM1 antibody (AA 65-385)

Catalog No. ABIN7602230

This anti-CARM1 antibody is a Rabbit Polyclonal antibody detecting CARM1 in WB and ELISA. Suitable for Human.

Quick Overview for CARM1 antibody (AA 65-385) (ABIN7602230)

Target: CARM1 (Coactivator-Associated Arginine Methyltransferase 1 (CARM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CARM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CARM1 Antibody

Binding Specificity: AA 65-385

Purpose: Anti-CARM1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CARM1 Antibody Picoband® (ABIN7602230). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CARM1 recombinant protein (Position: D65-H385).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chen D, Ma H, Hong H, Koh SS, Huang SM, Schurter BT, Aswad DW, Stallcup MR (1999). "Regulation of transcription by a protein methyltransferase". Science 284(5423): 2174-7. 2. El Messaoudi, S. E., Fabbrizio, E., Rodriguez, C., Chuchana, P., Fauquier, L., Cheng, D., Theillet, C., Vandel, L., Bedford, M. T., Sardet, C. Coactivator-associated arginine methyltransferase 1 (CARM1) is a positive regulator of the cyclin E1 gene. Proc. Nat. Acad. Sci. 103: 13351-13356, 2006. 3. Torres-Padilla ME, Parfitt DE, Kouzarides T, Zernicka-Goetz M (2007). "Histone arginine methylation regulates pluripotency in the early mouse embryo". Nature445 (7124): 214-8.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CARM1

Target: CARM1 (Coactivator-Associated Arginine Methyltransferase 1 (CARM1))

Alternative Name: CARM1

Background:

Synonyms: Flap endonuclease 1, FEN-1, DNase IV, Flap structure-specific endonuclease 1, Maturation factor 1, MF1, hFEN-1

Tissue Specificity: Predominantly expressed in the liver (PubMed:11099417, PubMed:11452359). Low expression levels in the small intestine and colon (PubMed:11099417). Very low levels in other tissues, including brain, heart and spleen (PubMed:11452359).

Background: CARM1(coactivator-associated arginine methyltransferase 1), also known as PRMT4, is an enzyme encoded by the CARM1 gene found in human beings, as well as many other mammals. This gene is mapped to 19p13.2. CARM1 is a regulator of cyclin E1 and DHFR mRNA expression. Its main function includes catalyzing the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. CARM1 is a secondary coactivator through its association with p160 family(SRC-1, GRIP1, AIB) of coactivators. It is responsible for moving cells toward the inner cell mass in developing blastocysts. This gene also plays an important role in androgen receptors and may play a role in prostate cancer progression.

Molecular Weight: 66 kDa

Gene ID: 10498

UniProt: Q86X55

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of fat Cell Differentiation