WDFY1 antibody (AA 65-394)

Catalog No. ABIN7602231

This Rabbit Polyclonal antibody specifically detects WDFY1 in ELISA, WB and FACS. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for WDFY1 antibody (AA 65-394) (ABIN7602231)

Target: WDFY1 (WD Repeat and FYVE Domain Containing 1 (WDFY1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WDFY1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-WDFY1 Antibody

Binding Specificity: AA 65-394

Purpose: Anti-FENS1/WDFY1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-FENS1/WDFY1 Antibody Picoband® (ABIN7602231). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human FENS1/WDFY1 recombinant protein (Position: H65-D394).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Dutta, S., Roy, S., Polavaram, N. S., Baretton, G. B., Muders, M. H., Batra, S., Datta, K. NRP2 transcriptionally regulates its downstream effector WDFY1. Sci. Rep. 6: 23588, 2016. Note: Electronic Article. 2. Gross, M. B. Personal Communication. Baltimore, Md. 8/8/2018. 3. Hu, Y.-H., Zhang, Y., Jiang, L.-Q., Wang, S., Lei, C.-Q., Sun, M.-S., Shu, H.-B., Liu, Y. WDFY1 mediates TLR3/4 signaling by recruiting TRIF. EMBO Rep. 16: 447-455, 2015.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for WDFY1

Target: WDFY1 (WD Repeat and FYVE Domain Containing 1 (WDFY1))

Alternative Name: WDFY1

Background:

Synonyms: Pre T-cell antigen receptor alpha, pT-alpha, pTa, pT-alpha-TCR, PTCRA

Tissue Specificity: Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.

Background: The protein encoded by this gene is a phosphatidylinositol 3-phosphate binding protein, which contains a FYVE zinc finger domain and multiple WD-40 repeat domains. When exogenously expressed, it localizes to early endosomes. Mutagenesis analysis demonstrates that this endosomal localization is mediated by the FYVE domain.

Molecular Weight: 46 kDa

Gene ID: 57590