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MELK antibody (AA 66-629)

This anti-MELK antibody is a Rabbit Polyclonal antibody detecting MELK in WB, ELISA, IHC, IF, ICC and FACS. Suitable for Human, Mouse and Rat.
Catalog No. ABIN7602251

Quick Overview for MELK antibody (AA 66-629) (ABIN7602251)

Target

See all MELK Antibodies
MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Reactivity

  • 64
  • 20
  • 16
  • 4
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
Human, Mouse, Rat

Host

  • 66
  • 7
Rabbit

Clonality

  • 64
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Polyclonal

Conjugate

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  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This MELK antibody is un-conjugated

Application

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  • 13
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  • 13
  • 9
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  • 4
  • 3
  • 1
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  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
  • Binding Specificity

    • 15
    • 8
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    • 4
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    • 2
    • 2
    • 2
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    • 1
    • 1
    • 1
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    • 1
    AA 66-629

    Purpose

    Anti-MELK Antibody Picoband®

    Cross-Reactivity (Details)

    No cross-reactivity with other proteins.

    Characteristics

    Anti-MELK Antibody Picoband® (ABIN7602251). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Purification

    Immunogen affinity purified.

    Immunogen

    E.coli-derived human MELK recombinant protein (Position: H66-Q629).

    Isotype

    IgG
  • Application Notes

    Western blot, 0.25-0.5 μg/mL, Human
    Immunohistochemistry, 2-5 μg/mL, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg /1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -
    1. Heyer, B. S., Warsowe, J., Solter, D., Knowles, B. B., Ackerman, S. L. New member of the Snf1/AMPK kinase family, Melk, is expressed in the mouse egg and preimplantation embryo. Molec. Reprod. Dev. 47: 148-156, 1997. 2. Hirose, T., Horvitz, H. R. An Sp1 transcription factor coordinates caspase-dependent and -independent apoptotic pathways. Nature 500: 354-358, 2013. 3. Nagase, T., Seki, N., Ishikawa, K., Tanaka, A., Nomura, N. Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1. DNA Res. 3: 17-24, 1996.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Concentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

    Storage

    4 °C,-20 °C

    Storage Comment

    At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
  • Target

    MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

    Alternative Name

    MELK

    Background

    Synonyms: Homeobox protein Hox-A5, Homeobox protein Hox-1C, HOXA5, HOX1C

    Tissue Specificity: Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. In the kidney, it is widely expressed in tubules, but sparsely expressed in the glomerulus (PubMed:24676636). Expression is significantly increased in renal biopsy specimens from idiopathic FSGS (PubMed:24676636). Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.

    Background: Maternal embryonic leucine zipper kinase (MELK) is an enzyme that in humans is encoded by the MELK gene. Maternal embryonic leucine-zipper kinase (MELK) is a key regulator of survival of stemlike GBM cells in vitro. MELK expression is increased in breast cancer tissue and this increase is also associated with poor patient survival, as predicted for a candidate oncogene.

    Molecular Weight

    66 kDa

    Gene ID

    9833

    UniProt

    Q14680
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