DGKZ antibody (AA 664-928)

Catalog No. ABIN7602261

This Rabbit Polyclonal antibody specifically detects DGKZ in WB, IHC, ELISA and FACS. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for DGKZ antibody (AA 664-928) (ABIN7602261)

Target: DGKZ (Diacylglycerol Kinase, zeta (DGKZ))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DGKZ antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-DGKZ Antibody

Binding Specificity: AA 664-928

Purpose: Anti-DGKZ/DGK-zeta Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-DGKZ/DGK-zeta Antibody Picoband® (ABIN7602261). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DGKZ/DGK-zeta recombinant protein (Position: E664-V928).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bunting, M., Tang, W., Zimmerman, G. A., McIntyre, T. M., Prescott, S. M. Molecular cloning and characterization of a novel human diacylglycerol kinase. J. Biol. Chem. 271: 10230-10236, 1996. 2. Gross, M. B. Personal Communication. Baltimore, Md. 4/11/2014. 3. Topham, M. K., Bunting, M., Zimmerman, G. A., McIntyre, T. M., Blackshear, P. J., Prescott, S. M. Protein kinase C regulates the nuclear localization of diacylglycerol kinase-zeta. Nature 394: 697-700, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for DGKZ

Target: DGKZ (Diacylglycerol Kinase, zeta (DGKZ))

Alternative Name: DGKZ

Background:

Synonyms: Ubiquitin carboxyl-terminal hydrolase 21, Deubiquitinating enzyme 21, Ubiquitin thioesterase 21, Ubiquitin-specific-processing protease 21, USP21, USP23, PP1490

Tissue Specificity: Highly expressed in heart, pancreas and skeletal muscle. Also expressed in brain, placenta, liver and kidney, and at very low level in lung.

Background: Diacylglycerol kinase zeta is an enzyme that in humans is encoded by the DGKZ gene. The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and multiple transcript variants encoding distinct isoforms have been identified.

Molecular Weight: 124 kDa

Gene ID: 8525

UniProt: Q13574

Pathways: Myometrial Relaxation and Contraction