SYVN1 antibody (AA 68-604)

Catalog No. ABIN7602291

This Rabbit Polyclonal antibody specifically detects SYVN1 in WB, ELISA, FACS, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SYVN1 antibody (AA 68-604) (ABIN7602291)

Target: SYVN1 (Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYVN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-SYVN1 Antibody

Binding Specificity: AA 68-604

Purpose: Anti-HRD1/SYVN1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-HRD1/SYVN1 Antibody Picoband® (ABIN7602291). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HRD1/SYVN1 recombinant protein (Position: Q68-R604).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Amano, T., Yamasaki, S., Yagishita, N., Tsuchimochi, K., Shin, H., Kawahara, K., Aratani, S., Fujita, H., Zhang, L., Ikeda, R., Fujii, R., Miura, N., Komiya, S., Nishioka, K., Maruyama, I., Fukamizu, A., Nakajima, T. Synoviolin/Hrd1, an E3 ubiquitin ligase, as a novel pathogenic factor for arthropathy. Genes Dev. 17: 2436-2449, 2003. 2. Burr, M. L., Cano, F., Svobodova, S., Boyle, L. H., Boname, J. M., Lehner, P. J. HRD1 and UBE2J1 target misfolded MHC class I heavy chains for endoplasmic reticulum-associated degradation. Proc. Nat. Acad. Sci. 108: 2034-2039, 2011. 3. Kaneko, M., Ishiguro, M., Niinuma, Y., Uesugi, M., Nomura, Y. Human HRD1 protects against ER stress-induced apoptosis through ER-associated degradation. FEBS Lett. 532: 147-152, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SYVN1

Target: SYVN1 (Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1))

Alternative Name: SYVN1

Background:

Synonyms: Dermatopontin, Tyrosine-rich acidic matrix protein, TRAMP, DPT

Tissue Specificity: Expressed in fibroblasts, heart, skeletal muscle, brain and pancreas. Expressed at an intermediate level in lung and kidney, and at a low level in liver and placenta. Expressed at a lower level in fibroblasts from hypertrophic scar lesional skin and in fibroblasts from patients with systemic sclerosis than in normal skin fibroblasts.

Background: E3 ubiquitin-protein ligase synoviolin is an enzyme that in humans is encoded by the SYVN1 gene. This gene encodes a protein involved in endoplasmic reticulum (ER)-associated degradation. The encoded protein removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. This protein also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins. Sequence analysis identified two transcript variants that encode different isoforms.

Molecular Weight: 76 kDa

Gene ID: 84447

Pathways: ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling