NAPRT1 antibody (AA 69-480)

Catalog No. ABIN7602305

This anti-NAPRT1 antibody is a Rabbit Polyclonal antibody detecting NAPRT1 in WB and ELISA. Suitable for Human.

Quick Overview for NAPRT1 antibody (AA 69-480) (ABIN7602305)

Target: NAPRT1 (NAPRT) (Nicotinate phosphoribosyltransferase (NAPRT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAPRT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NAPRT1 Antibody

Binding Specificity: AA 69-480

Purpose: Anti-NAPRT Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NAPRT Antibody Picoband® (ABIN7602305). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NAPRT recombinant protein (Position: A69-Q480).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chowdhry, S., Zanca, C., Rajkumar, U., Koga, T., Diao, Y., Raviram, R., Liu, F., Turner, K., Yang, H., Brunk, E., Bi, J., Furnari, F., Bafna, V., Ren, B., Mischel, P. S. NAD metabolic dependency in cancer is shaped by gene amplification and enhancer remodelling. Nature 569: 570-575, 2019. 2. Hara, N., Yamada, K., Shibata, T., Osago, H., Hashimoto, T., Tsuchiya, M. Elevation of cellular NAD levels by nicotinic acid and involvement of nicotinic acid phosphoribosyltransferase in human cells. J. Biol. Chem. 282: 24574-24582, 2007.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NAPRT1

Target: NAPRT1 (NAPRT) (Nicotinate phosphoribosyltransferase (NAPRT))

Alternative Name: NAPRT

Background:

Synonyms: Neutrophil defensin 1,Defensin, alpha 1,HNP-1,HP-1,HP1,HP 1-56,Neutrophil defensin 2,HNP-2,HP-2,HP2,DEFA1,DEF1, DEFA2, MRS,DEFA1B,

Tissue Specificity: Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.

Background: Nicotinic acid (NA, niacin) is converted by nicotinic acid phosphoribosyltransferase (NAPRT, EC 2.4.2.11) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD), which serves as a coenzyme in cellular redox reactions and is an essential component of a variety of processes in cellular metabolism including response to stress.

Molecular Weight: 58 kDa

Gene ID: 93100