POLR2D antibody (AA 7-124)

Catalog No. ABIN7602312

This Rabbit Polyclonal antibody specifically detects POLR2D in WB, ELISA, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for POLR2D antibody (AA 7-124) (ABIN7602312)

Target: POLR2D (Polymerase (RNA) II (DNA Directed) Polypeptide D (POLR2D))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2D antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-POLR2D Antibody

Binding Specificity: AA 7-124

Purpose: Anti-POLR2D Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-POLR2D Antibody Picoband® (ABIN7602312). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human POLR2D recombinant protein (Position: D7-D124).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Khazak, V., Estojak, J., Cho, H., Majors, J., Sonoda, G., Testa, J. R., Golemis, E. A. Analysis of the interaction of the novel RNA polymerase II (pol II) subunit hsRPB4 with its partner hsRPB7 and with pol II. Molec. Cell. Biol. 18: 1935-1945, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for POLR2D

Target: POLR2D (Polymerase (RNA) II (DNA Directed) Polypeptide D (POLR2D))

Alternative Name: POLR2D

Background:

Synonyms: Mediator of RNA polymerase II transcription subunit 9, Mediator complex subunit 9, MED9, MED25,

Tissue Specificity: Expressed in fetal brain, fetal lung, fetal liver, heart, brain, placenta, lung, liver, muscle, kidney and pancreas.

Background: DNA-ed RNA polymerase II subunit RPB4 is an enzyme that in humans is encoded by the POLR2D gene. This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene.

Molecular Weight: 18 kDa

Gene ID: 5433

UniProt: O15514

Pathways: Regulatory RNA Pathways