POLR2D antibody (N-Term)

Catalog No. ABIN6264344

This Rabbit Polyclonal antibody specifically detects POLR2D in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for POLR2D antibody (N-Term) (ABIN6264344)

Target: POLR2D (Polymerase (RNA) II (DNA Directed) Polypeptide D (POLR2D))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2D antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-POLR2D Antibody

Binding Specificity: N-Term

Specificity: POLR2D Antibody detects endogenous levels of total POLR2D.

Predicted Reactivity: Pig,Bovine,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human POLR2D, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for POLR2D

Target: POLR2D (Polymerase (RNA) II (DNA Directed) Polypeptide D (POLR2D))

Alternative Name: POLR2D

Background:

Description: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity).

Gene: POLR2D

Molecular Weight: 16kDa

Gene ID: 5433

UniProt: O15514

Pathways: Regulatory RNA Pathways