TRIM24 antibody (AA 706-964)

Catalog No. ABIN7602358

The Mouse Monoclonal anti-TRIM24 antibody has been validated for WB, IHC, IF and ICC. It is suitable to detect TRIM24 in samples from Human.

Quick Overview for TRIM24 antibody (AA 706-964) (ABIN7602358)

Target: TRIM24 (Tripartite Motif Containing 24 (TRIM24))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRIM24 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 4G6C2

Product Details anti-TRIM24 Antibody

Binding Specificity: AA 706-964

Purpose: Anti-TRIM24 Antibody Picoband® (monoclonal, 4G6C2)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TRIM24 Antibody Picoband® (monoclonal, 4G6C2) (ABIN7602358). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TRIM24 recombinant protein (Position: A706-D964).

Isotype: IgG1

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
1. Beckstead, R., Ortiz, J. A., Sanchez, C., Prokopenko, S. N., Chambon, P., Losson, R., Bellen, H. J. Bonus, a Drosophila homolog of TIF1 proteins, interacts with nuclear receptors and can inhibit beta-FTZ-F1-dependent transcription. Molec. Cell 7: 753-765, 2001. 2. Ignat, M., Teletin, M., Tisserand, J., Khetchoumian, K., Dennefeld, C., Chambon, P., Losson, R., Mark, M. Arterial calcifications and increased expression of vitamin D receptor targets in mice lacking TIF1-alpha. Proc. Nat. Acad. Sci. 105: 2598-2603, 2008. 3. Khetchoumian, K., Teletin, M., Tisserand, J., Mark, M., Herquel, B., Ignat, M., Zucman-Rossi, J., Cammas, F., Lerouge, T., Thibault, C., Metzger, D., Chambon, P., Losson, R. Loss of Trim24 (Tif1-alpha) gene function confers oncogenic activity to retinoic acid receptor alpha. Nature Genet. 39: 1500-1506, 2007.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TRIM24

Target: TRIM24 (Tripartite Motif Containing 24 (TRIM24))

Alternative Name: TRIM24

Background:

Synonyms: Peptidyl-prolyl cis-trans isomerase B, PPIase B, CYP-S1, Cyclophilin B, Rotamase B, S-cyclophilin, SCYLP, PPIB, CYPB

Tissue Specificity: Expressed predominantly in placenta.

Background: Tripartite motif-containing 24 (TRIM24) also known as transcriptional intermediary factor 1α (TIF1α) is a protein that, in humans, is encoded by the TRIM24 gene. The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Molecular Weight: 130 kDa

Gene ID: 8805

UniProt: O15164