ETV5 antibody (AA 72-246)

Catalog No. ABIN7602370

The Rabbit Polyclonal anti-ETV5 antibody has been validated for WB, ELISA, FACS, ICC and IF. It is suitable to detect ETV5 in samples from Human, Mouse and Rat.

Quick Overview for ETV5 antibody (AA 72-246) (ABIN7602370)

Target: ETV5 (Ets Variant 5 (ETV5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ETV5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-ETV5 Antibody

Binding Specificity: AA 72-246

Purpose: Anti-ERM/Etv5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ERM/Etv5 Antibody Picoband® (ABIN7602370). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ERM/Etv5 recombinant protein (Position: D72-Q246).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chen, C., Ouyang, W., Grigura, V., Zhou, Q., Carnes, K., Lim, H., Zhao, G.-Q., Arber, S., Kurpios, N., Murphy, T. L., Cheng, A. M., Hassell, J. A., Chandrashekar, V., Hofmann, M.-C., Hess, R. A., Murphy, K. M. ERM is required for transcriptional control of the spermatogonial stem cell niche. (Letter) Nature 436: 1030-1034, 2005. 2. Lu, B. C., Cebrian, C., Chi, X., Kuure, S., Kuo, R., Bates, C. M., Arber, S., Hassell, J., MacNeil, L., Hoshi, M., Jain, S., Asai, N., Takahashi, M., Schmidt-Ott, K. M., Barasch, J., D'Agati, V., Costantini, F. Etv4 and Etv5 are required downstream of GDNF and Ret for kidney branching morphogenesis. Nature Genet. 41: 1295-1302, 2009. Note: Erratum: Nature Genet. 42: 361 only, 2010. 3. Monte, D., Baert, J. L., Defossez, P. A., de Launoit, Y., Stehelin, D. Molecular cloning and characterization of human ERM, a new member of the Ets family closely related to mouse PEA3 and ER81 transcription factors. Oncogene 9: 1397-1406, 1994.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ETV5

Target: ETV5 (Ets Variant 5 (ETV5))

Alternative Name: ETV5

Background:

Synonyms: Glutamate receptor ionotropic, NMDA 1, GluN1, Glutamate [NMDA] receptor subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, NMD-R1, GRIN1, NMDAR1

Tissue Specificity: Widely expressed, with strongest expression in placenta (at protein level). Secreted by synovial fibroblasts. Up- regulated in osteoarthritis and rheumatoid arthritis synovial fluids and cartilage as compared with non-arthritic (at protein level).

Background: Ets variant 5 (ETV5) (also named ERM transcription factor) is a transcription factor that in humans is encoded by the ETV5 gene. It is generated in Sertoli cells, which are found in the testes and play a crucial role in spermatogenesis. Its ortholog has been linked to both obesity and bipolar disorder.

Molecular Weight: 70 kDa

Gene ID: 2119

UniProt: P41161

Pathways: Synaptic Membrane, Skeletal Muscle Fiber Development