Alanine--tRNA Ligase, Mitochondrial (AARS-1) (AA 729-968) antibody

Catalog No. ABIN7602380

This anti- antibody is a Rabbit Polyclonal antibody detecting in ELISA, IF, WB, FACS and ICC. Suitable for Human.

Quick Overview for Alanine--tRNA Ligase, Mitochondrial (AARS-1) (AA 729-968) antibody (ABIN7602380)

Target: Alanine--tRNA Ligase, Mitochondrial (AARS-1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details

Binding Specificity: AA 729-968

Purpose: Anti-AlaRS/AARS1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-AlaRS/AARS1 Antibody Picoband® (ABIN7602380). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human AlaRS/AARS1 recombinant protein (Position: R729-N968).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chihade, J. W., Brown, J. R., Schimmel, P. R., Ribas de Pouplana, L. Origin of mitochondria in relation to evolutionary history of eukaryotic alanyl-tRNA synthetase. Proc. Nat. Acad. Sci. 97: 12153-12157, 2000. 2. Guo, M., Chong, Y. E., Beebe, K., Shapiro, R., Yang, X.-L., Schimmel, P. The C-Ala domain brings together editing and aminoacylation functions on one tRNA. Science 325: 744-747, 2009. Note: Erratum: Science 326: 46 only, 2009. 3. Guo, M., Chong, Y. E., Shapiro, R., Beebe, K., Yang, X.-L., Schimmel, P. Paradox of mistranslation of serine for alanine caused by AlaRS recognition dilemma. Nature 462: 808-812, 2009.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details

Target: Alanine--tRNA Ligase, Mitochondrial (AARS-1)

Alternative Name: AARS1

Background:

Synonyms: Fos-related antigen 1, FRA-1, FOSL1, FRA1

Tissue Specificity: Adipocytes.

Background: An aminoacyl-tRNA synthetase (aaRS or ARS), also called tRNA-ligase, is an enzyme that attaches the appropriate amino acid onto its corresponding tRNA. The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41 % identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.

Molecular Weight: 107 kDa

Gene ID: 16

UniProt: P49588