Alanine--tRNA Ligase, Mitochondrial (AARS-1) (AA 729-968) antibody
Quick Overview for Alanine--tRNA Ligase, Mitochondrial (AARS-1) (AA 729-968) antibody (ABIN7602380)
Target
Reactivity
Host
Clonality
Application
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Binding Specificity
- AA 729-968
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Purpose
- Anti-AlaRS/AARS1 Antibody
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Cross-Reactivity (Details)
- No cross-reactivity with other proteins.
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Characteristics
- Anti-AlaRS/AARS1 Antibody (ABIN7602380). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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Purification
- Immunogen affinity purified.
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Immunogen
- E.coli-derived human AlaRS/AARS1 recombinant protein (Position: R729-N968).
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Isotype
- IgG
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Application Notes
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Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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Concentration
- 500 μg/mL
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Buffer
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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Storage
- 4 °C,-20 °C
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Storage Comment
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- Alanine--tRNA Ligase, Mitochondrial (AARS-1)
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Alternative Name
- AARS1
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Background
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Synonyms: Fos-related antigen 1, FRA-1, FOSL1, FRA1
Tissue Specificity: Adipocytes.
Background: An aminoacyl-tRNA synthetase (aaRS or ARS), also called tRNA-ligase, is an enzyme that attaches the appropriate amino acid onto its corresponding tRNA. The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41 % identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.
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Molecular Weight
- 107 kDa
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Gene ID
- 16
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UniProt
- P49588
Target
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