KERA antibody (AA 73-115)

Catalog No. ABIN7602381

This Rabbit Polyclonal antibody specifically detects KERA in WB and ELISA. It exhibits reactivity toward Mouse and Rat.

Quick Overview for KERA antibody (AA 73-115) (ABIN7602381)

Target: KERA (Keratocan (KERA))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KERA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-KERA Antibody

Binding Specificity: AA 73-115

Purpose: Anti-Keratocan/KTN/Kera Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Keratocan/KTN/Kera Antibody Picoband® (ABIN7602381). Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Keratocan/KTN/Kera recombinant protein (Position: R73-K115).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Dudakova, L., Palos, M., Hardcastle, A. J., Liskova, P. Corneal endothelial findings in a Czech patient with compound heterozygous mutations in KERA. (Letter) Ophthalmic Genet. 35: 252-254, 2014. 2. Funderburgh, J. L., Perchellet, A. L., Swiergiel, J., Conrad, G. W., Justice, M. J. Keratocan (Kera), a corneal keratan sulfate proteoglycan, maps to the distal end of mouse chromosome 10. Genomics 52: 110-111, 1998. 3. Khan, A. O., Al-Saif, A., Kambouris, M. A novel KERA mutation associated with autosomal recessive cornea plana. Ophthal. Genet. 25: 147-152, 2004. Note: Erratum: Ophthal. Genet. 25: 289 only, 2004, Ophthal. Genet. 26: 195 only, 2005.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for KERA

Target: KERA (Keratocan (KERA))

Alternative Name: Kera

Background:

Synonyms: Protein Hook homolog 3, h-hook3, Hhk3, HOOK3

Tissue Specificity: Highly expressed in chromaffin cells of the adrenal medulla (at protein level). Detected in peripheral sympathetic ganglia (at protein level). Found in some paracrine cells in stomach and duodenum (at protein level).

Background: Keratocan (KTN) also known as keratan sulfate proteoglycan keratocan, is a protein that in humans is encoded by the KERA gene. The protein encoded by this gene is a keratan sulfate proteoglycan that is involved in corneal transparency. Defects in this gene are a cause of autosomal recessive cornea plana 2 (CNA2).

Molecular Weight: 41 kDa

Gene ID: 16545

UniProt: O35367

Pathways: Glycosaminoglycan Metabolic Process