RREB1 antibody (AA 732-1559)

Catalog No. ABIN7602395

This anti-RREB1 antibody is a Rabbit Polyclonal antibody detecting RREB1 in WB, ELISA and FACS. Suitable for Human.

Quick Overview for RREB1 antibody (AA 732-1559) (ABIN7602395)

Target: RREB1 (Ras Responsive Element Binding Protein 1 (RREB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RREB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-RREB1 Antibody

Binding Specificity: AA 732-1559

Purpose: Anti-RREB1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RREB1 Antibody Picoband® (ABIN7602395). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RREB1 recombinant protein (Position: K732-Q1559).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Carson, E. B., McMahon, M., Baylin, S. B., Nelkin, B. D. Ret gene silencing is associated with Raf-1-induced medullary thyroid carcinoma cell differentiation. Cancer Res. 55: 2048-2052, 1995. 2. Flajollet, S., Poras, I., Carosella, E. D., Moreau, P. RREB-1 is a transcriptional repressor of HLA-G. J. Immun. 183: 6948-6959, 2009. 3. Kent, O. A., Saha, M., Coyaud, E., Burston, H. E., Law, N., Dadson, K., Chen, S., Laurent, E. M., St-Germain, J., Sun, R. X., Matsumoto, Y., Cowen, J., and 10 others. Haploinsufficiency of RREB1 causes a Noonan-like RASopathy via epigenetic reprogramming of RAS-MAPK pathway genes. Nature Commun. 11: 4673, 2020.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RREB1

Target: RREB1 (Ras Responsive Element Binding Protein 1 (RREB1))

Alternative Name: RREB1

Background:

Synonyms: Fructose-bisphosphate aldolase B, Liver-type aldolase, ALDOB, ALDB

Tissue Specificity: Pancreas-specific (at protein level). Loss of expression is seen in ductal type pancreas cancers.

Background: Ras-responsive element-binding protein 1 is a protein that in humans is encoded by the RREB1 gene. The protein encoded by this gene is a zinc finger transcription factor that binds to RAS-responsive elements (RREs) of gene promoters. It has been shown that the calcitonin gene promoter contains an RRE and that the encoded protein binds there and increases expression of calcitonin, which may be involved in Ras/Raf-mediated cell differentiation. Multiple transcript variants encoding several different isoforms have been found for this gene.

Molecular Weight: 160 kDa

Gene ID: 6239

UniProt: Q92766