THEMIS antibody (AA 74-588)

Catalog No. ABIN7602405

This Rabbit Polyclonal antibody specifically detects THEMIS in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for THEMIS antibody (AA 74-588) (ABIN7602405)

Target: THEMIS (Thymocyte Selection Associated (THEMIS))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This THEMIS antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-THEMIS Antibody

Binding Specificity: AA 74-588

Purpose: Anti-THEMIS Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-THEMIS Antibody Picoband® (ABIN7602405). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human THEMIS recombinant protein (Position: Q74-H588).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Fu, G., Casas, J., Rigaud, S., Rybakin, V., Lambolez, F., Brzostek, J., Hoerter, J. A. H., Paster, W., Acuto, O., Cheroutre, H., Sauer, K., Gascoigne, N. R. J. Themis sets the signal threshold for positive and negative selection in T-cell development. Nature 504: 441-445, 2013. 2. Fu, G., Vallee, S., Rybakin, V., McGuire, M. V., Ampudia, J., Brockmeyer, C., Salek, M., Fallen, P. R., Hoerter, J. A. H., Munshi, A., Huang, Y. H., Hu, J., Fox, H. S., Sauer, K., Acuto, O., Gascoigne, N. R. J. Themis controls thymocyte selection through regulation of T cell antigen receptor-mediated signaling. Nature Immun. 10: 848-856, 2009. 3. Johnson, A. L., Aravind, L., Shulzhenko, N., Morgun, A., Choi, S.-Y., Crockford, T. L., Lambe, T., Domaschenz, H., Kucharska, E. M., Zheng, L., Vinuesa, C. G., Lenardo, M. J., Goodnow, C. C., Cornall, R. J., Schwartz, R. H. Themis is a member of a new metazoan gene family and is required for the completion of thymocyte positive selection. Nature Immun. 10: 831-839, 2009. Note: Erratum: Nature Immun. 11: 97 only, 2010.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for THEMIS

Target: THEMIS (Thymocyte Selection Associated (THEMIS))

Alternative Name: THEMIS

Background:

Synonyms: Fascin-2, Retinal fascin, FSCN2

Tissue Specificity: Localized specifically in the outer and inner segments of the photoreceptor cells in the retina.

Background: This gene encodes a protein that plays a regulatory role in both positive and negative T-cell selection during late thymocyte development. The protein functions through T-cell antigen receptor signaling, and is necessary for proper lineage commitment and maturation of T-cells. Alternative splicing results in multiple transcript variants.

Molecular Weight: 73 kDa

Gene ID: 387357

UniProt: Q8N1K5