RPH3A antibody (AA 78-564)

Catalog No. ABIN7602450

This Rabbit Polyclonal antibody specifically detects RPH3A in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RPH3A antibody (AA 78-564) (ABIN7602450)

Target: RPH3A (Rabphilin 3A (RPH3A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPH3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-RPH3A Antibody

Binding Specificity: AA 78-564

Purpose: Anti-RPH3A Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RPH3A Antibody Picoband® (ABIN7602450). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RPH3A recombinant protein (Position: R78-Q564).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Inagaki, N., Mizuta, M., Seino, S. Cloning of a mouse rabphilin-3A expressed in hormone-secreting cells. J. Biochem. 116: 239-242, 1994. 2. Lin, C.-C., Huang, C.-C., Lin, K.-H., Cheng, K.-H., Yang, D.-M., Tsai, Y.-S., Ong, R.-Y., Huang, Y.-N., Kao, L.-S. Visualization of Rab3A dissociation during exocytosis: a study by total internal reflection microscopy. J. Cell. Physiol. 211: 316-326, 2007. 3. Nagase, T., Ishikawa, K., Suyama, M., Kikuno, R., Hirosawa, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 6: 63-70, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RPH3A

Target: RPH3A (Rabphilin 3A (RPH3A))

Alternative Name: RPH3A

Background:

Synonyms: Pre T-cell antigen receptor alpha, pT-alpha, pTa, pT-alpha-TCR, PTCRA

Tissue Specificity: Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.

Background: Rabphilin-3A is a protein that in humans is encoded by the RPH3A gene. The protein encoded by this gene is thought to be an effector for RAB3A, which is a small G protein that acts in the late stages of neurotransmitter exocytosis. The encoded protein may be involved in neurotransmitter release and synaptic vesicle traffic.

Molecular Weight: 85 kDa

Gene ID: 22895

UniProt: Q9Y2J0

Pathways: Synaptic Vesicle Exocytosis