OPLAH antibody (AA 788-1072)

Catalog No. ABIN7602454

The Rabbit Polyclonal anti-OPLAH antibody has been validated for WB, ELISA, FACS, ICC and IF. It is suitable to detect OPLAH in samples from Human, Mouse and Rat.

Quick Overview for OPLAH antibody (AA 788-1072) (ABIN7602454)

Target: OPLAH (5-Oxoprolinase (ATP-Hydrolysing) (OPLAH))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OPLAH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-OPLAH Antibody

Binding Specificity: AA 788-1072

Purpose: Anti-OPLAH Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-OPLAH Antibody Picoband® (ABIN7602454). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human OPLAH recombinant protein (Position: H788-E1072).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Almaghlouth, I. A., Mohamed, J. Y., Al-Amoudi, M., Al-Ahaidib, L., Al-Odaib, A., Alkuraya, F. S. 5-Oxoprolinase deficiency: report of the first human OPLAH mutation. Clin. Genet. 82: 193-196, 2012. 2. Calpena, E., Casado, M., Martinez-Rubio, D., Nascimento, A., Colomer, J., Gargallo, E., Garcia-Cazorla, A., Palau, F., Artuch, R., Espinos, C. 5-Oxoprolinuria in heterozygous patients for 5-oxoprolinase (OPLAH) missense changes. JIMD Rep. 7: 123-128, 2013. 3. Hartz, P. A. Personal Communication. Baltimore, Md. 9/22/2011.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for OPLAH

Target: OPLAH (5-Oxoprolinase (ATP-Hydrolysing) (OPLAH))

Alternative Name: OPLAH

Background:

Synonyms: Protein eva-1 homolog A, Protein FAM176A, Transmembrane protein 166, EVA1A, FAM176A, TMEM166, SP24

Tissue Specificity: Expressed in lung, kidney, liver, pancreas, placenta, but not in heart and skeletal muscle.

Background: The protein encoded by this gene acts as a homodimer, using ATP hydrolysis to catalyze the conversion of 5-oxo-L-proline to L-glutamate. Defects in this gene are a cause of 5-oxoprolinase deficiency (OPLAHD).

Molecular Weight: 140 kDa

Gene ID: 26873

UniProt: O14841