Laminin gamma 1 antibody (AA 79-1381)

Catalog No. ABIN7602455

This anti-Laminin gamma 1 antibody is a Rabbit Polyclonal antibody detecting Laminin gamma 1 in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for Laminin gamma 1 antibody (AA 79-1381) (ABIN7602455)

Target: Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Laminin gamma 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Laminin gamma 1 Antibody

Binding Specificity: AA 79-1381

Purpose: Anti-Laminin gamma 1/LAMC1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Laminin gamma 1/LAMC1 Antibody Picoband® (ABIN7602455). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Laminin gamma 1/LAMC1 recombinant protein (Position: Q79-K1381).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Burgeson, R. E., Chiquet, M., Deutzmann, R., Ekblom, P., Engel, J., Kleinman, H., Martin, G. R., Meneguzzi, G., Paulsson, M., Sanes, J., Timpl, R., Tryggvason, K., Yamada, Y., Yurchenco, P. D.A new nomenclature for the laminins.Matrix Biol. 14: 209-211, 1994. 2. Nikolova, G., Lee, H., Berkovitz, S., Nelson, S., Sinsheimer, J., Vilain, E., Rodriguez, L. V.Sequence variant in the laminin gamma-1 (LAMC1) gene associated with familial pelvic organ prolapse.Hum. Genet. 120: 847-856, 2007. 3. Smyth, N., Vatansever, H. S., Murray, P., Meyer, M., Frie, C., Paulsson, M., Edgar, D.Absence of basement membranes after targeting the LAMC1 gene results in embryonic lethality due to failure of endoderm differentiation.J. Cell Biol. 144: 151-160, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Laminin gamma 1

Target: Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

Alternative Name: LAMC1

Background:

Synonyms: Laminin subunit gamma-1,Laminin B2 chain,Laminin-1 subunit gamma,Laminin-10 subunit gamma,Laminin-11 subunit gamma,Laminin-2 subunit gamma,Laminin-3 subunit gamma,Laminin-4 subunit gamma,Laminin-6 subunit gamma,Laminin-7 subunit gamma,Laminin-8 subunit gamma,Laminin-9 subunit gamma,S-laminin subunit gamma,S-LAM gamma,Lamc1,Lamb-2, Lamc-1,

Tissue Specificity: Found in the basement membranes (major component).

Background: Laminin gamma1, Laminin subunit gamma-1, is a protein that in humans is encoded by the LAMC1 gene. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma(formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 1. The gamma 1 chain, formerly though to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of this gene also suggested that it had diverged considerably from the beta chain genes. Embryos of transgenic mice in which both alleles of the gamma 1 chain gene were inactivated by homologous recombination, lacked basement membranes, indicating that laminin, gamma 1 chain is necessary for laminin heterotrimer assembly. It has been inferred by analogy with the strikingly similar 3' UTR sequence in mouse laminin gamma 1 cDNA, that multiple polyadenylation sites are utilized in human to generate the 2 different sized mRNAs(5.5 and 7.5 kb) seen on Northern analysis.

Molecular Weight: 200 kDa

Gene ID: 3915

UniProt: P11047