BRDT antibody (AA 795-947)

Catalog No. ABIN7602468

This Rabbit Polyclonal antibody specifically detects BRDT in WB, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for BRDT antibody (AA 795-947) (ABIN7602468)

Target: BRDT (Bromodomain, Testis-Specific (BRDT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRDT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-BRDT Antibody

Binding Specificity: AA 795-947

Purpose: Anti-BRDT Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-BRDT Antibody Picoband® (ABIN7602468). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human BRDT recombinant protein (Position: Q795-D947).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gaucher, J., Boussouar, F., Montellier, E., Curtet, S., Buchou, T., Bertrand, S., Hery, P., Jounier, S., Depaux, A., Vitte, A.-L., Guardiola, P., Pernet, K., Debernardi, A., Lopez, F., Holota, H., Imbert, J., Wolgemuth, D. J., Gerard, M., Rousseaux, S., Khochbin, S. Bromodomain-dependent stage-specific male genome programming by Brdt. EMBO J. 31: 3809-3820, 2012. 2. Jones, M. H., Numata, M., Shimane, M. Identification and characterization of BRDT: a testis-specific gene related to the bromodomain genes RING3 and Drosophila fsh. Genomics 45: 529-534, 1997. 3. Li, L., Sha, Y., Wang, X., Li, P., Wang, J., Kee, K., Wang, B. Whole-exome sequencing identified a homozygous BRDT mutation in a patient with acephalic spermatozoa. Oncotarget 8: 19914-19922, 2017.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for BRDT

Target: BRDT (Bromodomain, Testis-Specific (BRDT))

Alternative Name: BRDT

Background:

Synonyms: Transmembrane protein 107,TMEM107,DC20, UNQ638/PRO1268,

Tissue Specificity: Ubiquitous. .

Background: Bromodomain testis-specific protein is a protein that in humans is encoded by the BRDT gene. BRDT is similar to the RING3 protein family. It possesses 2 bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain is found in proteins that regulate transcription. Several transcript variants encoding multiple isoforms have been found for this gene.

Molecular Weight: 180 kDa

Gene ID: 676

UniProt: Q58F21