POLDIP2 antibody (AA 81-324)

Catalog No. ABIN7602525

This Rabbit Polyclonal antibody specifically detects POLDIP2 in WB, ELISA, IF, FACS, ICC and IP. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for POLDIP2 antibody (AA 81-324) (ABIN7602525)

Target: POLDIP2 (Polymerase (DNA-Directed), delta Interacting Protein 2 (POLDIP2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLDIP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-POLDIP2 Antibody

Binding Specificity: AA 81-324

Purpose: Anti-POLDIP2 Antibody Picoband®

Characteristics: Anti-POLDIP2 Antibody Picoband® (ABIN7602525). Tested in ELISA, Flow Cytometry, IP, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human POLDIP2 recombinant protein (Position: Q81-Q324). Human POLDIP2 shares 99.2% amino acid (aa) sequence identity with mouse POLDIP2.

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Klaile, E., Muller, M. M., Kannicht, C., Otto, W., Singer, B. B., Reutter, W., Obrink, B., Lucka, L. The cell adhesion receptor carcinoembryonic antigen-related cell adhesion molecule 1 regulates nucleocytoplasmic trafficking of DNA polymerase delta-interacting protein 38. J. Biol. Chem. 282: 26629-26640, 2007. 2. Liu, L., Rodriguez-Belmonte, E. M., Mazloum, N., Xie, B., Lee, M. Y. W. T. Identification of a novel protein, PDIP38, that interacts with the p50 subunit of DNA polymerase delta and proliferating cell nuclear antigen. J. Biol. Chem. 278: 10041-10047, 2003. 3. Xie, B., Li, H., Wang, Q., Xie, S., Rahmeh, A., Dai, W., Lee, M. Y. W. T. Further characterization of human DNA polymerase delta interacting protein 38. J. Biol. Chem. 280: 22375-22384, 2005.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for POLDIP2

Target: POLDIP2 (Polymerase (DNA-Directed), delta Interacting Protein 2 (POLDIP2))

Alternative Name: POLDIP2

Background: Polymerase delta-interacting protein 2 also known as Polymerase delta-interacting protein of 38 kDa (PDIP38) is encoded by the POLDIP2 gene in humans. This gene encodes a protein that interacts with the DNA polymerase delta p50 subunit, as well as with proliferating cell nuclear antigen. The encoded protein maybe play a role in the ability of the replication fork to bypass DNA lesions. Alternative splicing results in multiple transcript variants.

Molecular Weight: 36 kDa

Gene ID: 26073

UniProt: Q9Y2S7