SPACA3 antibody (AA 86-215)

Catalog No. ABIN7602569

Product Details anti-SPACA3 Antibody

Target: SPACA3 (Sperm Acrosome Associated Protein 3 (SPACA3))

Binding Specificity: AA 86-215

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPACA3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purpose: Anti-SPACA3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SPACA3 Antibody Picoband® (ABIN7602569). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SPACA3 recombinant protein (Position: E86-F215).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Mandal, A., Klotz, K. L., Shetty, J., Jayes, F. L., Wolkowicz, M. J., Bolling, L. C., Coonrod, S. A., Black, M. B., Diekman, A. B., Haystead, T. A. J., Flickinger, C. J., Herr, J. C. SLLP1, a unique, intra-acrosomal, non-bacteriolytic, c lysozyme-like protein of human spermatozoa. Biol. Reprod. 68: 1525-1537, 2003. 2. Zhang, K., Gao, R., Zhang, H., Cai, X., Shen, C., Wu, C., Zhao, S., Yu, L. Molecular cloning and characterization of three novel lysozyme-like genes, predominantly expressed in the male reproductive system of humans, belonging to the C-type lysozyme/alpha-lactalbumin family. Biol. Reprod. 73: 1064-1071, 2005.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SPACA3

Target: SPACA3 (Sperm Acrosome Associated Protein 3 (SPACA3))

Alternative Name: SPACA3 (SPACA3 Products)

Background:

Synonyms: Pre T-cell antigen receptor alpha, pT-alpha, pTa, pT-alpha-TCR, PTCRA

Tissue Specificity: Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.

Background: Sperm acrosome membrane-associated protein 3 is a protein that in humans is encoded by the SPACA3 gene. The protein encoded by this gene is a sperm surface protein that may be involved in adhesion to the egg prior to fertilization. While the encoded protein has significant similarity to lysozyme at the amino acid level, it has no detectable bacteriocidal activity. Several transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 40 kDa

Gene ID: 124912

Pathways: Glycosaminoglycan Metabolic Process