Nebulette antibody (AA 86-842)

Catalog No. ABIN7602577

The Rabbit Polyclonal anti-Nebulette antibody has been validated for WB, ELISA and IHC. It is suitable to detect Nebulette in samples from Human, Mouse and Rat.

Quick Overview for Nebulette antibody (AA 86-842) (ABIN7602577)

Target: Nebulette (NEBL)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nebulette antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-Nebulette Antibody

Binding Specificity: AA 86-842

Purpose: Anti-NEBL Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NEBL Antibody Picoband® (ABIN7602577). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NEBL recombinant protein (Position: E86-K842).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Arimura, T., Nakamura, T., Hiroi, S., Satoh, M., Takahashi, M., Ohbuchi, N., Ueda, K., Nouchi, T., Yamaguchi, N., Akai, J., Matsumori, A., Sasayama, S., Kimura, A. Characterization of the human nebulette gene: a polymorphism in an actin-binding motif is associated with nonfamilial idiopathic dilated cardiomyopathy. Hum. Genet. 107: 440-451, 2000. 2. Li, B., Zhuang, L., Trueb, B. Zyxin interacts with the SH3 domains of the cytoskeletal proteins LIM-nebulette and Lasp-1. J. Biol. Chem. 279: 20401-20410, 2004. 3. Millevoi, S., Trombitas, K., Kolmerer, B., Kostin, S., Schaper, J., Pelin, K., Granzier, H., Labeit, S. Characterization of nebulette and nebulin and emerging concepts of their roles for vertebrate Z-discs. J. Molec. Biol. 282: 111-123, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Nebulette

Target: Nebulette (NEBL)

Alternative Name: NEBL

Background:

Synonyms: Protein phosphatase 1E, Ca (2+)/calmodulin-dependent protein kinase phosphatase N, CaMKP-N, CaMKP-nucleus, CaMKN, Partner of PIX 1, Partner of PIX-alpha, Partner of PIXA, PPM1E, CAMKN, KIAA1072, POPX1

Tissue Specificity: Expressed in fetal and adult brain. Also detected in fetal liver and skeletal muscle, but not in their adult counterparts.

Background: Nebulette is a cardiac-specific isoform belonging to the nebulin family of proteins. It is encoded by the NEBL gene. This gene encodes a nebulin like protein that is abundantly expressed in cardiac muscle. The encoded protein binds actin and interacts with thin filaments and Z-line associated proteins in striated muscle. This protein may be involved in cardiac myofibril assembly. A shorter isoform of this protein termed LIM nebulette is expressed in non-muscle cells and may function as a component of focal adhesion complexes. Alternate splicing results in multiple transcript variants.

Molecular Weight: 126 kDa

Gene ID: 10529

UniProt: O76041