POMP antibody (AA 9-141)

Catalog No. ABIN7602614

This anti-POMP antibody is a Rabbit Polyclonal antibody detecting POMP in WB, ELISA and FACS. Suitable for Human.

Quick Overview for POMP antibody (AA 9-141) (ABIN7602614)

Target: POMP (Proteasome Maturation Protein (POMP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POMP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-POMP Antibody

Binding Specificity: AA 9-141

Purpose: Anti-POMP Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-POMP Antibody Picoband® (ABIN7602614). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human POMP recombinant protein (Position: E9-L141).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Brehm, A., Liu, Y., Sheikh, A., Marrero, B., Omoyinmi, E., Zhou, Q., Montealegre, G., Biancotto, A., Reinhardt, A., Almeida de Jesus, A., Pelletier, M., Tsai, W. L., and 31 others. Additive loss-of-function proteasome subunit mutations in CANDLE/PRAAS patients promote type I IFN production. J. Clin. Invest. 125: 4196-4211, 2015. Note: Erratum: J. Clin. Invest. 126: 795 only, 2016. 2. Chondrogianni, N., Gonos, E. S. Overexpression of hUMP1/POMP proteasome accessory protein enhances proteasome-mediated antioxidant defence. Exp. Gerontol. 42: 899-903, 2007. 3. Dahlqvist, J., Klar, J., Tiwari, N., Schuster, J., Torma, H., Badhai, J., Pujol, R., van Steensel, M. A. M., Brinkhuizen, T., Gijezen, L., Chaves, A., Tadini, G., Vahlquist, A., Dahl, N. A single-nucleotide deletion in the POMP 5-prime UTR causes a transcriptional switch and altered epidermal proteasome distribution in KLICK genodermatosis. Am. J. Hum. Genet. 86: 596-603, 2010. Note: Erratum: Am. J. Hum. Genet. 86: 655 only, 2010.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for POMP

Target: POMP (Proteasome Maturation Protein (POMP))

Alternative Name: POMP

Background:

Synonyms: NACHT, LRR and PYD domains-containing protein 2, Nucleotide-binding site protein 1, PYRIN domain and NACHT domain-containing protein 1, PYRIN-containing APAF1-like protein 2, NLRP2, NALP2, NBS1, PAN1, PYPAF2

Tissue Specificity: Expressed at high levels in lung, placenta and thymus and at lower levels in ovary, intestine and brain.

Background: Proteasome maturation protein is a protein that in humans is encoded by the POMP gene. The protein encoded by this gene is a molecular chaperone that binds 20S preproteasome components and is essential for 20S proteasome formation. The 20S proteasome is the proteolytically active component of the 26S proteasome complex. The encoded protein is degraded before the maturation of the 20S proteasome is complete. A variant in the 5' UTR of this gene has been associated with KLICK syndrome, a rare skin disorder.

Molecular Weight: 16 kDa

Gene ID: 51371

UniProt: Q9Y244