MAFA antibody (AA 9-308)

Catalog No. ABIN7602623

This anti-MAFA antibody is a Rabbit Polyclonal antibody detecting MAFA in WB, ELISA, IHC, IF, ICC and FACS. Suitable for Human and Mouse.

Quick Overview for MAFA antibody (AA 9-308) (ABIN7602623)

Target: MAFA (Transcription Factor MafA (MAFA))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAFA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-MAFA Antibody

Binding Specificity: AA 9-308

Purpose: Anti-MAFA Picoband® Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MAFA Picoband® Antibody (ABIN7602623). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MAFA recombinant protein (Position: A9-D308).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Iacovazzo, D., Flanagan, S. E., Walker, E., Quezado, R., de Sousa Barros, F. A., Caswell, R., Johnson, M. B., Wakeling, M., Brandle, M., Guo, M., Dang, M. N., Gabrovska, P., and 14 others. MAFA missense mutation causes familial insulinomatosis and diabetes mellitus. Proc. Nat. Acad. Sci. 115: 1027-1032, 2018. 2. Olbrot, M., Rud, J., Moss, L. G., Sharma, A. Identification of beta-cell-specific insulin gene transcription factor RIPE3b1 as mammalian MafA. Proc. Nat. Acad. Sci. 99: 6737-6742, 2002. 3. Tragl, K.-H., Mayr, W. R. Familial islet-cell adenomatosis. Lancet 318: 426-428, 1977. Note: Originally Volume II.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MAFA

Target: MAFA (Transcription Factor MafA (MAFA))

Alternative Name: MAFA

Background:

Synonyms: Transcription factor MafA, Pancreatic beta-cell-specific transcriptional activator, RIPE3b1 factor, V-maf musculoaponeurotic fibrosarcoma oncogene homolog A, MAFA

Tissue Specificity: Preferentially expressed in regulatory T-cells (Tregs).

Background: MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene. It is mapped to 8q24.3.

Molecular Weight: 50 kDa

Gene ID: 389692

Pathways: Hormone Transport