LIMA1 antibody (AA 9-744)

Catalog No. ABIN7602639

This Rabbit Polyclonal antibody specifically detects LIMA1 in WB, ELISA, IHC, IF, ICC and FACS. It exhibits reactivity toward Human.

Quick Overview for LIMA1 antibody (AA 9-744) (ABIN7602639)

Target: LIMA1 (LIM Domain and Actin Binding 1 (LIMA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIMA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-LIMA1 Antibody

Binding Specificity: AA 9-744

Purpose: Anti-EPLIN/LIMA1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-EPLIN/LIMA1 Antibody Picoband® (ABIN7602639). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human EPLIN/LIMA1 recombinant protein (Position: R9-E744).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chen, S., Maul, R. S., Kim, H. R., Chang, D. D. Characterization of the human EPLIN (Epithelial Protein Lost in Neoplasm) gene reveals distinct promoters for the two EPLIN isoforms. Gene 248: 69-76, 2000. 2. Maul, R. S., Chang, D. D. EPLIN, epithelial protein lost in neoplasm. Oncogene 18: 7838-7841, 1999. 3. Maul, R. S., Song, Y., Amann, K. J., Gerbin, S. C., Pollard, T. D., Chang, D. D. EPLIN regulates actin dynamics by cross-linking and stabilizing filaments. J. Cell Biol. 160: 399-407, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for LIMA1

Target: LIMA1 (LIM Domain and Actin Binding 1 (LIMA1))

Alternative Name: LIMA1

Background:

Synonyms: Zinc finger protein Helios, Ikaros family zinc finger protein 2, IKZF2, HELIOS, ZNFN1A2

Tissue Specificity: Ubiquitous. High expression in liver, spleen, pancreas and kidney.

Background: LIM domain and actin-binding protein 1 is a protein that in humans is encoded by the LIMA1 gene. This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated.

Molecular Weight: 85 kDa, 100 kDa

Gene ID: 51474