Lipin 1 antibody (AA 90-886)

Catalog No. ABIN7602648

This Rabbit Polyclonal antibody specifically detects Lipin 1 in WB, ELISA, ICC, IF and FACS. It exhibits reactivity toward Human and Rat.

Quick Overview for Lipin 1 antibody (AA 90-886) (ABIN7602648)

Target: Lipin 1 (LPIN1)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lipin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Lipin 1 Antibody

Binding Specificity: AA 90-886

Purpose: Anti-Lipin 1/LPIN1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Lipin 1/LPIN1 Antibody Picoband® (ABIN7602648). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Lipin 1/LPIN1 recombinant protein (Position: Q90-H886).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Cao, H., Hegele, R. A. Identification of single-nucleotide polymorphisms in the human LPIN1 gene. J. Hum. Genet. 47: 370-372, 2002. 2. Han, G.-S., Wu, W.-I., Carman, G. M. The Saccharomyces cerevisiae lipin homolog is a Mg(2+)-dependent phosphatidate phosphatase enzyme. J. Biol. Chem. 281: 9210-9218, 2006. 3. Han, S., Bahmanyar, S., Zhang, P., Grishin, N., Oegema, K., Crooke, R., Graham, M., Reue, K., Dixon, J. E., Goodman, J. M. Nuclear envelope phosphatase 1-regulatory subunit 1 (formerly TMEM188) is the metazoan Spo7p ortholog and functions in the lipin activation pathway. J. Biol. Chem. 287: 3123-3137, 2012.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Lipin 1

Target: Lipin 1 (LPIN1)

Alternative Name: LPIN1

Background:

Synonyms: Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Vasa homolog, DDX4, VASA

Tissue Specificity: Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.

Background: This gene encodes a magnesium-ion-dependent phosphatidic acid phosphohydrolase enzyme that catalyzes the penultimate step in triglyceride synthesis including the dephosphorylation of phosphatidic acid to yield diacylglycerol. Expression of this gene is required for adipocyte differentiation and it also functions as a nuclear transcriptional coactivator with some peroxisome proliferator-activated receptors to modulate expression of other genes involved in lipid metabolism. Mutations in this gene are associated with metabolic syndrome, type 2 diabetes, acute recurrent rhabdomyolysis, and autosomal recessive acute recurrent myoglobinuria (ARARM). This gene is also a candidate for several human lipodystrophy syndromes.

Molecular Weight: 130 kDa

Gene ID: 23175

UniProt: Q14693

Pathways: Monocarboxylic Acid Catabolic Process