NCAPH antibody (AA 91-721)

Catalog No. ABIN7602660

This Rabbit Polyclonal antibody specifically detects NCAPH in WB, ELISA, IF, ICC and FACS. It exhibits reactivity toward Human.

Quick Overview for NCAPH antibody (AA 91-721) (ABIN7602660)

Target: NCAPH (Non-SMC Condensin I Complex, Subunit H (NCAPH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCAPH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-NCAPH Antibody

Binding Specificity: AA 91-721

Purpose: Anti-NCAPH Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NCAPH Antibody Picoband® (ABIN7602660). Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NCAPH recombinant protein (Position: R91-K721).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Aono, N., Sutani, T., Tomonaga, T., Mochida, S., Yanagida, M. Cnd2 has dual roles in mitotic condensation and interphase. Nature 417: 197-202, 2002. 2. Bhat, M. A., Philp, A. V., Glover, D. M., Bellen, H. J. Chromatid segregation at anaphase requires the barren product, a novel chromosome-associated protein that interacts with topoisomerase II. Cell 87: 1103-1114, 1996. 3. Cabello, O. A., Baldini, A., Bhat, M., Bellen, H., Belmont, J. W. Localization of BRRN1, the human homologue of Drosophila barr, to 2q11.2. Genomics 46: 311-313, 1997.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NCAPH

Target: NCAPH (Non-SMC Condensin I Complex, Subunit H (NCAPH))

Alternative Name: NCAPH

Background:

Synonyms: Mediator of RNA polymerase II transcription subunit 20, Mediator complex subunit 20, TRF-proximal protein homolog, hTRFP, MED20, TRFP,

Tissue Specificity: Pre-B-cells and B-cells but not terminally differentiated plasma cells.

Background: Condensin complex subunit 2 also known as chromosome-associated protein H (CAP-H) or non-SMC condensin I complex subunit H (NCAPH) is a protein that in humans is encoded by the NCAPH gene. This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization. Alternatively spliced transcript variants encoding different proteins have been described.

Molecular Weight: 100 kDa

Gene ID: 23397

UniProt: Q15003